Nomenclature: CXCR5

Family: Chemokine receptors

Annotation status:  image of a green circle Annotated and expert reviewed. Please contact us if you can help with updates. 

Contents

Gene and Protein Information
class A G protein-coupled receptor
Species TM AA Chromosomal Location Gene Symbol Gene Name Reference
Human 7 372 11q23.3 CXCR5 chemokine (C-X-C motif) receptor 5 3
Mouse 7 374 9 A5.2 Cxcr5 chemokine (C-X-C motif) receptor 5 8
Rat 7 374 8q22 Cxcr5 chemokine (C-X-C motif) receptor 5 10
Previous and Unofficial Names
Names References
NLR 10
MDR15 1
BLR-1 4,11
BLR1
CD185
Burkitt lymphoma receptor 1, GTP-binding protein
Burkitt lymphoma receptor 1, GTP binding protein (chemokine (C-X-C motif) receptor 5)
Gpcr6
Burkitt lymphoma receptor 1
C-X-C chemokine receptor type 5
CXC-R5
CXCR-5
burkitt lymphoma receptor 1 homolog
neurolymphatic receptor
chemokine (C-X-C motif) receptor 5
Database Links
Ensembl Gene
Entrez Gene
GPCRDB
GeneCards
GenitoUrinary Development Molecular Anatomy Project
HomoloGene
Human Protein Reference Database
InterPro
KEGG Gene
OMIM
PharmGKB Gene
PhosphoSitePlus
Protein Ontology (PRO)
RefSeq Nucleotide
RefSeq Protein
TreeFam
UniGene Hs.
UniProtKB
Wikipedia
Natural/Endogenous Ligands
CXCL13 {Sp: Human} , CXCL13 {Sp: Mouse}
Agonist Comments
It has been shown that the main ligand for the CXCR5 receptor is CXCL13 (BLC, BCA-1) [11]
Primary Transduction Mechanisms
Transducer Effector/Response
Gi/Go family Calcium channel
References: 
Tissue Distribution
Glomerular podocytes.
Species:  Human
Technique:  RT-PCR.
References:  6
Blood and cerebrospinal fluid B cells.
Species:  Human
Technique:  Flow cytometry.
References:  16
Peripheral blood follicular helper T cells (TFH) (subset of CD4+ T cells), CD8+ T cells, B cells.
Species:  Human
Technique:  Flow cytometry.
References:  14
B and T cells in gut associated lymphoid tissue.
Species:  Human
Technique:  Immunofluorescence.
References:  2
Peripheral blood and tonsillar T cells.
Species:  Human
Technique:  Flow cytometry.
References:  15
Bone marrow stromal cells (BMSCs).
Species:  Human
Technique:  Flow cytometry and RT-PCR.
References:  5
Osteoblasts.
Species:  Human
Technique:  RT-PCR and flow cytometry.
References:  12
Expression Datasets

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Log average relative transcript abundance in mouse tissues measured by qPCR from Regard, J.B., Sato, I.T., and Coughlin, S.R. (2008). Anatomical profiling of G protein-coupled receptor expression. Cell, 135(3): 561-71. [PMID:18984166] [Raw data: website]

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Functional Assays
Measurement of chemotaxis of blood B lymphocytes endogenously expressing the CXCR5 receptor.
Species:  Human
Tissue:  Blood B lymphocytes.
Response measured:  Chemotaxis.
References:  11
Measurement of Ca2+ levels in murine pre-B cells transfected with the human CXCR5 receptor.
Species:  Human
Tissue:  Murine pre-B cells 300-19.
Response measured:  Ca2+ mobilisation.
References:  11
Detection of HIV-2 entry into NP-2 cells transfected with the human CXCR5 receptor and CD4.
Species:  Human
Tissue:  NP-2 cell line.
Response measured:  HIV-2 entry.
References:  9
Physiological Functions
Chemotaxis.
Species:  Human
Tissue:  Blood B lymphocytes.
References:  11
Organogenesis (cooperative with the CCR7 receptor).
Species:  Mouse
Tissue:  Lymphoid organ.
References:  13
B cell migration.
Species:  Mouse
Tissue:  In vivo (spleen).
References:  4
Physiological Consequences of Altering Gene Expression
CXCR5-/- CCR7-/- double knockout mice lack peripheral lymph nodes.
Species:  Mouse
Tissue: 
Technique:  Gene targeting in embryonic stem cells.
References:  13
CXCR5 receptor knockout mice and CXCR5-/- CCR7-/- double knockout mice exhibit decreased B cell levels in the peritoneal cavity as well as reduced IgM responses to administration of Streptococcus pneumoniae.
Species:  Mouse
Tissue: 
Technique:  Gene targeting in embryonic stem cells.
References:  7
CXCR5 receptor knockout mice lack inguinal lymph nodes, have fewer and abnormal Peyer's patches, impaired migration of lymphocytes into splenic follicles causing altered primary lymphoid follicles, and no functional germinal center development in the spleen.
Species:  Mouse
Tissue: 
Technique:  Gene targeting in embryonic stem cells.
References:  4
Phenotypes, Alleles and Disease Models Mouse data from MGI

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Allele Composition & genetic background Accession Phenotype Id Phenotype Reference
Cxcr5tm1Lipp Cxcr5tm1Lipp/Cxcr5tm1Lipp
involves: 129S2/SvPas * CD-1
MGI:103567  MP:0003156 abnormal leukocyte migration PMID: 8978608 
Cxcr5tm1Lipp Cxcr5tm1Lipp/Cxcr5tm1Lipp
involves: 129S2/SvPas * CD-1
MGI:103567  MP:0000696 abnormal Peyer's patch morphology PMID: 8978608 
Cxcr5tm1Lipp Cxcr5tm1Lipp/Cxcr5tm1Lipp
involves: 129S2/SvPas * CD-1
MGI:103567  MP:0008471 abnormal spleen primary B follicle morphology PMID: 8978608 
Cxcr5tm1Lipp Cxcr5tm1Lipp/Cxcr5tm1Lipp
involves: 129S2/SvPas * CD-1
MGI:103567  MP:0008472 abnormal spleen secondary B follicle morphology PMID: 8978608 
Cxcr5tm1Lipp Cxcr5tm1Lipp/Cxcr5tm1Lipp
involves: 129S2/SvPas * CD-1
MGI:103567  MP:0009630 absent axillary lymph nodes PMID: 10917533 
Cxcr5tm1Lipp Cxcr5tm1Lipp/Cxcr5tm1Lipp
involves: 129S2/SvPas * CD-1
MGI:103567  MP:0009628 absent brachial lymph nodes PMID: 10917533 
Cxcr5tm1Lipp Cxcr5tm1Lipp/Cxcr5tm1Lipp
involves: 129S2/SvPas * CD-1
MGI:103567  MP:0009633 absent cervical lymph nodes PMID: 10917533 
Cxcr5tm1Lipp Cxcr5tm1Lipp/Cxcr5tm1Lipp
involves: 129S2/SvPas * CD-1
MGI:103567  MP:0008201 absent follicular dendritic cells PMID: 10917533 
Cxcr5tm1Lipp Cxcr5tm1Lipp/Cxcr5tm1Lipp
involves: 129S2/SvPas * CD-1
MGI:103567  MP:0009622 absent inguinal lymph nodes PMID: 10917533  8978608 
Cxcr5tm1Lipp Cxcr5tm1Lipp/Cxcr5tm1Lipp
involves: 129S2/SvPas * CD-1
MGI:103567  MP:0008024 absent lymph nodes PMID: 10917533 
Cxcr5tm1Lipp Cxcr5tm1Lipp/Cxcr5tm1Lipp
involves: 129S2/SvPas * CD-1
MGI:103567  MP:0002831 absent Peyer's patches PMID: 8978608 
Cxcr5tm1Lipp Cxcr5tm1Lipp/Cxcr5tm1Lipp
involves: 129S2/SvPas * CD-1
MGI:103567  MP:0008474 absent spleen germinal center PMID: 8978608 
Cxcr5tm1Lipp Cxcr5tm1Lipp/Cxcr5tm1Lipp
involves: 129S2/SvPas * CD-1
MGI:103567  MP:0005014 increased B cell number PMID: 8978608 
Available Assays
DiscoveRx PathHunter® C2C12 CXCR5 β-Arrestin Cell Line (Cat no. 93-0204C7)
PathHunter® CHO-K1 CXCR5 β-Arrestin Cell Line (Cat no. 93-0514C2)
PathHunter® eXpress CXCR5 CHO-K1 β-Arrestin GPCR Assay (Cat no. 93-0514E2CP2M)
more info

REFERENCES

1. Barella L, Loetscher M, Tobler A, Baggiolini M, Moser B. (1995) Sequence variation of a novel heptahelical leucocyte receptor through alternative transcript formation. Biochem J309: 773-779. [PMID:7639692]

2. Carlsen HS, Baekkevold ES, Johansen FE, Haraldsen G, Brandtzaeg P. (2002) B cell attracting chemokine 1 (CXCL13) and its receptor CXCR5 are expressed in normal and aberrant gut associated lymphoid tissue. Gut51: 364-371. [PMID:12171958]

3. Dobner T, Wolf I, Emrich T, Lipp M. (1992) Differentiation-specific expression of a novel G protein-coupled receptor from Burkitt's lymphoma. Eur J Immunol22: 2795-2799. [PMID:1425907]

4. Forster R, Mattis AE, Kremmer E, Wolf E, Brem G, Lipp M. (1996) A putative chemokine receptor, BLR1, directs B cell migration to defined lymphoid organs and specific anatomic compartments of the spleen. Cell87: 1037-1047. [PMID:8978608]

5. Honczarenko M, Le Y, Swierkowski M, Ghiran I, Glodek A, Silberstein LE. (2005) HUMAN BONE MARROW STROMAL CELLS EXPRESS A DISTINCT SET OF BIOLOGICALLY FUNCTIONAL CHEMOKINE RECEPTORS. Stem Cells, : -. [PMID:16253981]

6. Huber TB, Reinhardt HC, Exner M, Burger JA, Kerjaschki D, Saleem MA, Pavenstädt H. (2002) Expression of functional CCR and CXCR chemokine receptors in podocytes. J Immunol168: 6244-6252. [PMID:12055238]

7. Höpken UE, Achtman AH, Krüger K, Lipp M. (2004) Distinct and overlapping roles of CXCR5 and CCR7 in B-1 cell homing and early immunity against bacterial pathogens. J Leukoc Biol76: 709-718. [PMID:15197239]

8. Kaiser E, Förster R, Wolf I, Ebensperger C, Kuehl WM, Lipp M. (1993) The G protein-coupled receptor BLR1 is involved in murine B cell differentiation and is also expressed in neuronal tissues. Eur J Immunol23: 2532-2539. [PMID:8405054]

9. Kanbe K, Shimizu N, Soda Y, Takagishi K, Hoshino H. (1999) A CXC chemokine receptor, CXCR5/BLR1, is a novel and specific coreceptor for human immunodeficiency virus type 2. Virology265: 264-273. [PMID:10600598]

10. Kouba M, Vanetti M, Wang X, Schäfer M, Höllt V. (1993) Cloning of a novel putative G-protein-coupled receptor (NLR) which is expressed in neuronal and lymphatic tissue. FEBS Lett321: 173-178. [PMID:8386678]

11. Legler DF, Loetscher M, Roos RS, Clark-Lewis I, Baggiolini M, Moser B. (1998) B cell-attracting chemokine 1, a human CXC chemokine expressed in lymphoid tissues, selectively attracts B lymphocytes via BLR1/CXCR5. J Exp Med187: 655-660. [PMID:9463416]

12. Lisignoli G, Toneguzzi S, Piacentini A, Cattini L, Lenti A, Tschon M, Cristino S, Grassi F, Facchini A. (2003) Human osteoblasts express functional CXC chemokine receptors 3 and 5: activation by their ligands, CXCL10 and CXCL13, significantly induces alkaline phosphatase and beta-N-acetylhexosaminidase release. J Cell Physiol194: 71-79. [PMID:12447991]

13. Ohl L, Henning G, Krautwald S, Lipp M, Hardtke S, Bernhardt G, Pabst O, Förster R. (2003) Cooperating mechanisms of CXCR5 and CCR7 in development and organization of secondary lymphoid organs. J Exp Med197: 1199-1204. [PMID:12732661]

14. Saito R, Onodera H, Tago H, Suzuki Y, Shimizu M, Matsumura Y, Kondo T, Itoyama Y. (2005) Altered expression of chemokine receptor CXCR5 on T cells of myasthenia gravis patients. J Neuroimmunol170: 172-178. [PMID:16214223]

15. Schaerli P, Willimann K, Lang AB, Lipp M, Loetscher P, Moser B. (2000) CXC chemokine receptor 5 expression defines follicular homing T cells with B cell helper function. J Exp Med192: 1553-1562. [PMID:11104798]

16. Sørensen TL, Roed H, Sellebjerg F. (2002) Chemokine receptor expression on B cells and effect of interferon-beta in multiple sclerosis. J Neuroimmunol122: 125-131. [PMID:11777551]

To cite this database page, please use the following:

Israel F. Charo, Rebecca Hills, Richard Horuk, Kouji Matsushima, Philip M. Murphy, Joost J. Oppenheim.
Chemokine receptors: CXCR5. Last modified on 15/08/2013. Accessed on 30/09/2014. IUPHAR database (IUPHAR-DB), http://www.iuphar-db.org/DATABASE/ObjectDisplayForward?objectId=72.

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