Nomenclature: CCR7

Family: Chemokine receptors

Annotation status:  image of a green circle Annotated and expert reviewed. Please contact us if you can help with updates. 

Contents

Gene and Protein Information
class A G protein-coupled receptor
Species TM AA Chromosomal Location Gene Symbol Gene Name Reference
Human 7 378 17q12-q21.2 CCR7 chemokine (C-C motif) receptor 7 12
Mouse 7 378 11 D Ccr7 chemokine (C-C motif) receptor 7 12
Rat 7 378 10q31 Ccr7 chemokine (C-C motif) receptor 7
Previous and Unofficial Names
Names References
EBI-1 12,16
BLR-2
CMKBR7
EBI1
BLR2
CDw197
CD197
MGC108519
C-C chemokine receptor type 7
chemokine (C-C motif) receptor 7
Ebi1h
Database Links
ChEMBL Target
Ensembl Gene
Entrez Gene
GPCRDB
GeneCards
GenitoUrinary Development Molecular Anatomy Project
HomoloGene
Human Protein Reference Database
InterPro
KEGG Gene
OMIM
PharmGKB Gene
PhosphoSitePlus
Protein Ontology (PRO)
RefSeq Nucleotide
RefSeq Protein
TreeFam
UniGene Hs.
UniProtKB
Wikipedia
Natural/Endogenous Ligands
CCL19 {Sp: Human} , CCL19 {Sp: Mouse}
CCL21 {Sp: Human}
Agonists
Key to terms and symbols Click column headers to sort
Ligand Sp. Action Affinity Units Reference
CCL21 {Sp: Human} Hs Full agonist 9.3 pIC50 17
pIC50 9.3 [17]
CCL19 {Sp: Human} Hs Full agonist 7.7 – 9.0 pIC50 16-17
pIC50 7.7 – 9.0 [16-17]
Primary Transduction Mechanisms
Transducer Effector/Response
Gi/Go family Calcium channel
References:  11,13-14
Secondary Transduction Mechanisms
Transducer Effector/Response
Gi/Go family Other - See Comments
Comments:  Activation of the MAPK cascade.
References:  7
Tissue Distribution
T cells.
Species:  Human
Technique:  Multicolor flow cytometry.
References:  6
Bone marrow stromal cells (BMSCs).
Species:  Human
Technique:  Flow cytometry and RT-PCR.
References:  3
Natural killer (NK) cells.
Species:  Human
Technique:  Flow cytometric and RNase protection assay.
References:  5
Blood T lymphocytes.
Species:  Human
Technique:  Flow cytometry.
References:  1
Monocytes.
Species:  Mouse
Technique:  RT-PCR.
References:  10
Mature dendritic cells.
Species:  Mouse
Technique:  Western blotting.
References:  15
T cells.
Species:  Mouse
Technique:  Microarray analysis, RNase protection assay, Western blotting and in vitro chemokine transmigration assays.
References:  8
Expression Datasets

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Log average relative transcript abundance in mouse tissues measured by qPCR from Regard, J.B., Sato, I.T., and Coughlin, S.R. (2008). Anatomical profiling of G protein-coupled receptor expression. Cell, 135(3): 561-71. [PMID:18984166] [Raw data: website]

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Functional Assays
Measurement of Ca2+ levels in K562 cells transfected with the human CCR7 receptor.
Species:  Human
Tissue:  K562 cells.
Response measured:  Ca2+ mobilisation.
References:  16
Measurement of chemotaxis of HEK 293/EBNA-1 cells transfected with the human CCR7 receptor.
Species:  Human
Tissue:  HEK 293/EBNA-1 cells.
Response measured:  Chemotaxis.
References:  16
Measurement of Ca2+ levels in murine L1.2 cells transfected with the human CCR7 receptor.
Species:  Human
Tissue:  Murine L1.2 cells.
Response measured:  Ca2+ mobilisation.
References:  17
Measurement of chemotaxis of murine L1.2 cells transfected with the human CCR7 receptor.
Species:  Human
Tissue:  Murine L1.2 cells.
Response measured:  Chemotaxis.
References:  17
Measurement of the phosphorylation of MAPKs (ERK1/2, JNK, and p38) in dendritic cells endogenously expressing the CCR7 receptor.
Species:  Human
Tissue:  Dendritic cells.
Response measured:  PTX-sensitive activation of ERK1/2, JNK, and p38.
References:  11
Measurement of MAP kinase activity in HEK 293 cells transfected with the human CCR7 receptor.
Species:  Human
Tissue:  HEK 293 cells.
Response measured:  Activation of ERK1/2.
References:  7
Physiological Functions
Organogenesis (cooperative with the CXCR5 receptor).
Species:  Mouse
Tissue:  Lymphoid organ.
References:  9
Antiapoptotic signalling.
Species:  Human
Tissue:  Dendritic cells.
References:  14
Physiological Consequences of Altering Gene Expression
CXCR5-/- CCR7-/- double knockout mice exhibit decreased B cell levels in the peritoneal cavity as well as reduced IgM responses to administration of Streptococcus pneumoniae.
Species:  Mouse
Tissue: 
Technique:  Gene targeting in embryonic stem cells.
References:  4
CCR7-/- CXCR5-/- double knockout mice lack peripheral lymph nodes.
Species:  Mouse
Tissue: 
Technique:  Gene targeting in embryonic stem cells.
References:  9
CCR7 receptor knockout mice exhibit profound disorganisation of secondary lymphoid tissue and defective primary immune responses.
Species:  Mouse
Tissue: 
Technique:  Gene targeting in embryonic stem cells.
References:  2
Phenotypes, Alleles and Disease Models Mouse data from MGI

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Allele Composition & genetic background Accession Phenotype Id Phenotype Reference
Ccr7tm1Rfor Ccr7tm1Rfor/Ccr7tm1Rfor
involves: 129P2/OlaHsd * BALB/c
MGI:103011  MP:0008217 abnormal B cell activation PMID: 10520991 
Ccr7tm1Rfor Ccr7tm1Rfor/Ccr7tm1Rfor
involves: 129P2/OlaHsd * BALB/c
MGI:103011  MP:0002459 abnormal B cell physiology PMID: 10520991 
Ccr7tm1Rfor|Tg(DO11.10)10Dlo Ccr7tm1Rfor/Ccr7tm1Rfor,Tg(DO11.10)10Dlo/?
involves: 129P2/OlaHsd
MGI:103011  MGI:2664401  MP:0003091 abnormal cell migration PMID: 17474155 
Ccr7tm1Rfor Ccr7tm1Rfor/Ccr7tm1Rfor
involves: 129P2/OlaHsd * BALB/c
MGI:103011  MP:0004816 abnormal class switch recombination PMID: 10520991 
Ccr7tm1Rfor Ccr7tm1Rfor/Ccr7tm1Rfor
involves: 129P2/OlaHsd * BALB/c
MGI:103011  MP:0002376 abnormal dendritic cell physiology PMID: 10520991 
Ccr7tm1Rfor Ccr7tm1Rfor/Ccr7tm1Rfor
involves: 129/Sv * 129P2/OlaHsd * C57BL/6
MGI:103011  MP:0002376 abnormal dendritic cell physiology PMID: 18451854 
Ccr7tm1Rfor Ccr7tm1Rfor/Ccr7tm1Rfor
B6.129P2-Ccr7
MGI:103011  MP:0002163 abnormal gland morphology PMID: 16473829 
Ccr7tm1Rfor Ccr7tm1Rfor/Ccr7tm1Rfor
involves: 129P2/OlaHsd * BALB/c
MGI:103011  MP:0001800 abnormal humoral immune response PMID: 10520991 
Ccr7tm1Rfor Ccr7tm1Rfor/Ccr7tm1Rfor
B6.129P2-Ccr7
MGI:103011  MP:0001346 abnormal lacrimal gland morphology PMID: 16473829 
Ccr7tm1Rfor Ccr7tm1Rfor/Ccr7tm1Rfor
involves: 129P2/OlaHsd * BALB/c
MGI:103011  MP:0003156 abnormal leukocyte migration PMID: 10520991 
Ccr7tm1Rfor|Tg(DO11.10)10Dlo Ccr7tm1Rfor/Ccr7tm1Rfor,Tg(DO11.10)10Dlo/?
involves: 129P2/OlaHsd
MGI:103011  MGI:2664401  MP:0003156 abnormal leukocyte migration PMID: 17474155 
Ccr7tm1Rfor Ccr7tm1Rfor/Ccr7tm1Rfor
B6.129P2-Ccr7
MGI:103011  MP:0003156 abnormal leukocyte migration PMID: 15302902  16473829 
Ccr7tm1Rfor Ccr7tm1Rfor/Ccr7tm1Rfor
involves: 129P2/OlaHsd * BALB/c
MGI:103011  MP:0002343 abnormal lymph node cortex PMID: 10520991 
Ccr7tm1Rfor Ccr7tm1Rfor/Ccr7tm1Rfor
involves: 129P2/OlaHsd * BALB/c
MGI:103011  MP:0008522 abnormal lymph node germinal center PMID: 10520991 
Ccr7tm1Rfor Ccr7tm1Rfor/Ccr7tm1Rfor
involves: 129P2/OlaHsd * BALB/c
MGI:103011  MP:0002347 abnormal lymph node T cell domain PMID: 10520991 
Ccr7tm1Rfor Ccr7tm1Rfor/Ccr7tm1Rfor
B6.129P2-Ccr7
MGI:103011  MP:0003452 abnormal parotid gland morphology PMID: 16473829 
Ccr7tm1Rfor Ccr7tm1Rfor/Ccr7tm1Rfor
involves: 129P2/OlaHsd * BALB/c
MGI:103011  MP:0002392 abnormal Peyer's patch T cell area PMID: 10520991 
Ccr7tm1Rfor Ccr7tm1Rfor/Ccr7tm1Rfor
B6.129P2-Ccr7
MGI:103011  MP:0008872 abnormal physiological response to xenobiotic PMID: 16473829 
Ccr7tm1Rfor|Tg(DO11.10)10Dlo Ccr7tm1Rfor/Ccr7tm1Rfor,Tg(DO11.10)10Dlo/?
involves: 129P2/OlaHsd
MGI:103011  MGI:2664401  MP:0004946 abnormal regulatory T cell physiology PMID: 17474155 
Ccr7tm1Rfor Ccr7tm1Rfor/Ccr7tm1Rfor
involves: 129P2/OlaHsd * BALB/c
MGI:103011  MP:0002363 abnormal spleen marginal sinus morphology PMID: 10520991 
Ccr7tm1Rfor Ccr7tm1Rfor/Ccr7tm1Rfor
involves: 129P2/OlaHsd * BALB/c
MGI:103011  MP:0002358 abnormal spleen periarteriolar lymphoid sheath morphology PMID: 10520991 
Ccr7tm1Rfor Ccr7tm1Rfor/Ccr7tm1Rfor
involves: 129P2/OlaHsd * BALB/c
MGI:103011  MP:0002356 abnormal spleen red pulp morphology PMID: 10520991 
Ccr7tm1Rfor Ccr7tm1Rfor/Ccr7tm1Rfor
B6.129P2-Ccr7
MGI:103011  MP:0003793 abnormal submandibular gland morphology PMID: 16473829 
Ccr7tm1Rfor Ccr7tm1Rfor/Ccr7tm1Rfor
involves: 129P2/OlaHsd * BALB/c
MGI:103011  MP:0002444 abnormal T cell physiology PMID: 10520991 
Ccr7tm1Rfor Ccr7tm1Rfor/Ccr7tm1Rfor
B6.129P2-Ccr7
MGI:103011  MP:0002371 abnormal thymus cortex morphology PMID: 15302902 
Ccr7tm1Rfor Ccr7tm1Rfor/Ccr7tm1Rfor
B6.129P2-Ccr7
MGI:103011  MP:0002375 abnormal thymus medulla morphology PMID: 15302902 
Ccr7tm1Dgen Ccr7tm1Dgen/Ccr7tm1Dgen
involves: 129P2/OlaHsd * C57BL/6
MGI:103011  MP:0002499 chronic inflammation
Ccr7tm1Rfor Ccr7tm1Rfor/Ccr7tm1Rfor
involves: 129P2/OlaHsd * BALB/c
MGI:103011  MP:0008075 decreased CD4-positive T cell number PMID: 10520991 
Ccr7tm1Rfor Ccr7tm1Rfor/Ccr7tm1Rfor
involves: 129P2/OlaHsd * BALB/c
MGI:103011  MP:0008127 decreased dendritic cell number PMID: 10520991 
Ccr7tm1Rfor Ccr7tm1Rfor/Ccr7tm1Rfor
involves: 129P2/OlaHsd * BALB/c
MGI:103011  MP:0008498 decreased IgG3 level PMID: 10520991 
Ccr7tm1Rfor Ccr7tm1Rfor/Ccr7tm1Rfor
involves: 129P2/OlaHsd * BALB/c
MGI:103011  MP:0005616 decreased susceptibility to type IV hypersensitivity reaction PMID: 10520991 
Ccr7tm1Rfor Ccr7tm1Rfor/Ccr7tm1Rfor
involves: 129P2/OlaHsd * BALB/c
MGI:103011  MP:0005018 decreased T cell number PMID: 10520991 
Ccr7Gt(hpk)Byg Ccr7Gt(hpk)Byg/Ccr7Gt(hpk)Byg
involves: 129 * C57BL/6
MGI:103011  MP:0003008 enhanced long term potentiation PMID: 9825957 
Ccr7tm1Rfor Ccr7tm1Rfor/Ccr7tm1Rfor
involves: 129P2/OlaHsd * BALB/c
MGI:103011  MP:0000691 enlarged spleen PMID: 10520991 
Ccr7tm1Rfor Ccr7tm1Rfor/Ccr7tm1Rfor
involves: 129P2/OlaHsd * BALB/c
MGI:103011  MP:0008074 increased CD4-positive T cell number PMID: 10520991 
Ccr7tm1Rfor Ccr7tm1Rfor/Ccr7tm1Rfor
involves: 129P2/OlaHsd * BALB/c
MGI:103011  MP:0002497 increased IgE level PMID: 10520991 
Ccr7tm1Rfor Ccr7tm1Rfor/Ccr7tm1Rfor
involves: 129P2/OlaHsd * BALB/c
MGI:103011  MP:0008499 increased IgG1 level PMID: 10520991 
Ccr7tm1Rfor Ccr7tm1Rfor/Ccr7tm1Rfor
involves: 129P2/OlaHsd * BALB/c
MGI:103011  MP:0008500 increased IgG2a level PMID: 10520991 
Ccr7tm1Rfor Ccr7tm1Rfor/Ccr7tm1Rfor
B6.129P2-Ccr7
MGI:103011  MP:0001846 increased inflammatory response PMID: 16473829 
Ccr7tm1Rfor Ccr7tm1Rfor/Ccr7tm1Rfor
B6.129P2-Ccr7
MGI:103011  MP:0005350 increased susceptibility to autoimmune disorder PMID: 16473829 
Ccr7tm1Rfor Ccr7tm1Rfor/Ccr7tm1Rfor
involves: 129P2/OlaHsd * BALB/c
MGI:103011  MP:0005015 increased T cell number PMID: 10520991 
Ccr7tm1Rfor Ccr7tm1Rfor/Ccr7tm1Rfor
B6.129P2-Ccr7
MGI:103011  MP:0008880 lacrimal gland inflammation PMID: 16473829 
Ccr7tm1Rfor Ccr7tm1Rfor/Ccr7tm1Rfor
B6.129P2-Ccr7
MGI:103011  MP:0001870 salivary gland inflammation PMID: 16473829 
Ccr7tm1Rfor Ccr7tm1Rfor/Ccr7tm1Rfor
involves: 129P2/OlaHsd * BALB/c
MGI:103011  MP:0002217 small lymph nodes PMID: 10520991 
Ccr7tm1Rfor Ccr7tm1Rfor/Ccr7tm1Rfor
involves: 129P2/OlaHsd * BALB/c
MGI:103011  MP:0008135 small Peyer's patches PMID: 10520991 
Available Assays
DiscoveRx PathHunter® CHO-K1 CCR7 β-Arrestin Cell Line (Cat no. 93-0195C2)
PathHunter® eXpress CCR7 CHO-K1 β-Arrestin GPCR Assay (Cat no. 93-0195E2CP0M)
PathHunter® eXpress HEK 293 CCR7 β-Arrestin GPCR Assay (Cat no. 93-0195E1CP0M)
PathHunter® HEK 293 CCR7 β-Arrestin Cell Line (Cat no. 93-0195C1)
more info

REFERENCES

1. Bardi G, Lipp M, Baggiolini M, Loetscher P. (2001) The T cell chemokine receptor CCR7 is internalized on stimulation with ELC, but not with SLC. Eur J Immunol31: 3291-3297. [PMID:11745346]

2. Forster R, Schubel A, Breitfield D, Kremmer E, Renner-Muller I, Wolf E, Lipp M. (1999) CCR7 coordinates the primary immune response by establishing functional microenvironments in secondary lymphoid organs. Cell99: 23-33. [PMID:10520991]

3. Honczarenko M, Le Y, Swierkowski M, Ghiran I, Glodek A, Silberstein LE. (2005) HUMAN BONE MARROW STROMAL CELLS EXPRESS A DISTINCT SET OF BIOLOGICALLY FUNCTIONAL CHEMOKINE RECEPTORS. Stem Cells, : -. [PMID:16253981]

4. Höpken UE, Achtman AH, Krüger K, Lipp M. (2004) Distinct and overlapping roles of CXCR5 and CCR7 in B-1 cell homing and early immunity against bacterial pathogens. J Leukoc Biol76: 709-718. [PMID:15197239]

5. Inngjerdingen M, Damaj B, Maghazachi AA. (2001) Expression and regulation of chemokine receptors in human natural killer cells. Blood97: 367-375. [PMID:11154210]

6. Kim JW, Ferris RL, Whiteside TL. (2005) Chemokine C receptor 7 expression and protection of circulating CD8+ T lymphocytes from apoptosis. Clin Cancer Res11: 7901-7910. [PMID:16278415]

7. Kohout TA, Nicholas SL, Perry SJ, Reinhart G, Junger S, Struthers RS. (2004) Differential desensitization, receptor phosphorylation, beta-arrestin recruitment, and ERK1/2 activation by the two endogenous ligands for the CC chemokine receptor 7. J Biol Chem279: 23214-23222. [PMID:15054093]

8. Mo R, Chen J, Han Y, Bueno-Cannizares C, Misek DE, Lescure PA, Hanash S, Yung RL. (2003) T cell chemokine receptor expression in aging. J Immunol170: 895-904. [PMID:12517955]

9. Ohl L, Henning G, Krautwald S, Lipp M, Hardtke S, Bernhardt G, Pabst O, Förster R. (2003) Cooperating mechanisms of CXCR5 and CCR7 in development and organization of secondary lymphoid organs. J Exp Med197: 1199-1204. [PMID:12732661]

10. Qu C, Edwards EW, Tacke F, Angeli V, Llodrá J, Sanchez-Schmitz G, Garin A, Haque NS, Peters W, van Rooijen N, Sanchez-Torres C, Bromberg J, Charo IF, Jung S, Lira SA, Randolph GJ. (2004) Role of CCR8 and other chemokine pathways in the migration of monocyte-derived dendritic cells to lymph nodes. J Exp Med200: 1231-1241. [PMID:15534368]

11. Riol-Blanco L, Sánchez-Sánchez N, Torres A, Tejedor A, Narumiya S, Corbí AL, Sánchez-Mateos P, Rodríguez-Fernández JL. (2005) The chemokine receptor CCR7 activates in dendritic cells two signaling modules that independently regulate chemotaxis and migratory speed. J Immunol174: 4070-4080. [PMID:15778365]

12. Schweickart VL, Raport CJ, Godiska R, Byers MG, Eddy RL, Shows TB, Gray PW. (1994) Cloning of human and mouse EBI1, a lymphoid-specific G-protein-coupled receptor encoded on human chromosome 17q12-q21.2. Genomics23: 643-650. [PMID:7851893]

13. Sullivan SK, McGrath DA, Grigoriadis D, Bacon KB. (1999) Pharmacological and signaling analysis of human chemokine receptor CCR-7 stably expressed in HEK-293 cells: high-affinity binding of recombinant ligands MIP-3beta and SLC stimulates multiple signaling cascades. Biochem Biophys Res Commun263: 685-690. [PMID:10512740]

14. Sánchez-Sánchez N, Riol-Blanco L, de la Rosa G, Puig-Kröger A, García-Bordas J, Martin D, Longo N, Cuadrado A, Cabañas C, Corbí AL, Sánchez-Mateos P, Rodríguez-Fernández JL. (2004) Chemokine receptor CCR7 induces intracellular signaling that inhibits apoptosis of mature dendritic cells. Blood104: 619-625. [PMID:15059845]

15. Walker SR, Aboka A, Ogagan PD, Barksdale EM. (2005) Murine neuroblastoma attenuates dendritic cell cysteine cysteine receptor 7 (CCR7) expression. J Pediatr Surg40: 983-987. [PMID:15991182]

16. Yoshida R, Imai T, Hieshima K, Kusuda J, Baba M, Kitaura M, Nishimura M, Kakizaki M, Nomiyama H, Yoshie O. (1997) Molecular cloning of a novel human CC chemokine EBI1-ligand chemokine that is a specific functional ligand for EBI1, CCR7. J Biol Chem272: 13803-13809. [PMID:9153236]

17. Yoshida R, Nagira M, Kitaura M, Imagawa N, Imai T, Yoshie O. (1998) Secondary lymphoid-tissue chemokine is a functional ligand for the CC chemokine receptor CCR7. J Biol Chem273: 7118-7122. [PMID:9507024]

To cite this database page, please use the following:

Israel F. Charo, Rebecca Hills, Richard Horuk, Kouji Matsushima, Philip M. Murphy, Joost J. Oppenheim.
Chemokine receptors: CCR7. Last modified on 31/01/2013. Accessed on 25/10/2014. IUPHAR database (IUPHAR-DB), http://www.iuphar-db.org/DATABASE/ObjectDisplayForward?objectId=64.

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