image of a green circle Annotated and expert reviewed. Please contact us if you can help with updates. 

Kv10.2

Family: Voltage-gated potassium channels

Contents:
Gene and Protein Information
Previous and Unofficial Names
Database Links
Ion Selectivity and Conductance
Voltage Dependence
Pore Blockers
Tissue Distribution
Phenotypes, Alleles and Disease Models
Biologically Significant Variants
General Comments
References
Gene and Protein Information
Species TM P Loops AA Chromosomal Location Gene Symbol Gene Name Reference
Human 6 1 988 14q23.1 KCNH5 potassium voltage-gated channel, subfamily H (eag-related), member 5
Mouse 6 1 988 12 C3 Kcnh5 potassium voltage-gated channel, subfamily H (eag-related), member 5
Rat 6 1 988 6q24 Kcnh5 potassium voltage-gated channel, subfamily H (eag-related), member 5
Previous and Unofficial Names
eag2
KCNH5
Kv10.2
H-EAG2
ether-a-go-go potassium channel 2
potassium voltage-gated channel subfamily H member 5
rEAG2
voltage-gated potassium channel subunit Kv10.2
Database Links
Ensembl ENSG00000140015 (Hs), ENSMUSG00000034402 (Mm), ENSRNOG00000009542 (Rn)
Entrez Gene 27133 (Hs), 238271 (Mm), 171146 (Rn)
GeneCards KCNH5 (Hs)
GenitoUrinary Development Molecular Anatomy Project Kcnh5 (Mm)
HomoloGene 15858 (Hs)
Human Protein Reference Database 10421 (Hs)
InterPro Q8NCM2 (Hs), Q920E3 (Mm), Q9EPI9 (Rn)
KEGG Gene hsa:27133 (Hs), mmu:238271 (Mm), rno:171146 (Rn)
OMIM 605716 (Hs)
PharmGKB Gene PA30040 (Hs)
PhosphoSitePlus Q8NCM2 (Hs), Q920E3 (Mm)
Protein Ontology (PRO) PRO:000000794 (Hs)
RefSeq Nucleotide NM_139318 (Hs), NM_172376 (Hs), NM_172375 (Hs), NM_172805 (Mm), NM_133610 (Rn)
RefSeq Protein NP_758963 (Hs), NP_758964 (Hs), NP_647479 (Hs), NP_766393 (Mm), NP_598294 (Rn)
TreeFam ENSG00000140015 (Hs), ENSMUSG00000034402 (Mm), ENSRNOG00000009542 (Rn)
UniGene Hs. 27043 (Hs)
UniProt Q8NCM2 (Hs), Q920E3 (Mm), Q9EPI9 (Rn)
Wikipedia Kv10.2
Potassium channels
Search for 3D structures on the PDB
Search by keyword: Voltage-gated potassium channels Kv10.2 Search by accession number: Q9EPI9, Q920E3, Q8NCM2
Ion Selectivity and Conductance
Species:  Rat
Macroscopic current rectification:  Outwardly rectifying, non-inactivating voltage dependent currents
References:  6
Voltage Dependence
  V0.5 (mV)  τ (msec)  Reference  Cell type  Species 
Activation  -100.0 - 6 Xenopus laevis oocyte Rat
Inactivation  - -
Associated subunits (Human)
minK
Pore Blockers
Key to terms and symbols View all chemical structures Click column headers to sort
Ligand Sp. Affinity Units Concentration range (M) Holding voltage (mV) Reference
LY97241 Rn 5.8 pIC50 - - 1,4
quinidine Rn 3.8 pIC50 - - 1
Pore Blocker Comments
Intracellular Calcium (Cai2+): At 20ηM Cai2+ current declined ~ 30% after 90 seconds. At 400 ηM Cai2+, only 10% of residual current left after 90 seconds [5]

Terfenadine pIC50 > 5 [3]
Tissue Distribution
Skeletal muscle, heart, placenta, lung, liver, kidney, pancreas
Species:  Human
Technique:  Northern Blot, RT-PCR
References:  3
Brain (layer IV of the cerebral cortex, thalamus, inferior colliculus, olfactory bulb, certain brainstem nuclei)
Species:  Rat
Technique:  Northern Blot, in situ hybridization
References:  5-6
Phenotypes, Alleles and Disease Models Mouse data from MGI

Click here to show/hide data

Allele Composition & genetic background Accession Phenotype Id Phenotype Reference
Kcnh5tm1Dgen Kcnh5tm1Dgen/Kcnh5tm1Dgen
involves: 129P2/OlaHsd * C57BL/6		 MGI:3584508  MP:0001486 abnormal startle reflex
Kcnh5tm1Dgen Kcnh5tm1Dgen/Kcnh5tm1Dgen
involves: 129P2/OlaHsd * C57BL/6		 MGI:3584508  MP:0002797 increased thigmotaxis
Biologically Significant Variants
Kv10.2 isoform 3
Nucleotide accession:  NM_172375 
Protein accession:  NP_758963 
Amino acids:  611
Type:  Splice variant
Species:  Human
References: 
Kv10.2 isoform 1
Nucleotide accession:  NM_139318 
Protein accession:  NP_647479 
Amino acids:  988
Type:  Splice variant
Species:  Human
References: 
Kv10.2 isoform 2
Nucleotide accession:  NM_172376 
Protein accession:  NP_758964 
Amino acids:  624
Type:  Splice variant
Species:  Human
References: 
Biologically Significant Variant Comments
The funtional significance of isoform-2 and -3 variants is yet to be established
General Comments

This channel has:

GFG (rather than the common GYG) potassium channel signature sequence,

a PAS domain in the distal part of the cytosolic N-terminus,

a cNBD domain in the proximal portion of the C-terminus,

a C-terminal assembly domain (CAD),

a CaM binding domain,

a bNLS domain in the C-terminus,

and a C-terminal domain required for assembly [4].

The tetramerizing coiled-coil (TCC) domain at the C-terminal end of Kv10 and Kv11 confers specificity for multimer formation, allowing Kv10.1 / Kv10.2 heteromerization, and Kv11.1 homomerization, but not Kv10.x / Kv11.1 heteromerization [2].

This C-terminal TCC domain has been identified in many other channels, and mutations of the TCC have been found to be linked to genetic channelopathies.

REFERENCES

Show »

1. Gessner, G; Zacharias, M; Bechstedt, S; Schönherr, R; Heinemann, SH. (2004) Molecular determinants for high-affinity block of human EAG potassium channels by antiarrhythmic agents. Mol. Pharmacol., 65 (5): 1120-9. [PMID:15102940]

2. Jenke, M; Sánchez, A; Monje, F; Stühmer, W; Weseloh, RM; Pardo, LA. (2003) C-terminal domains implicated in the functional surface expression of potassium channels. EMBO J., 22 (3): 395-403. [PMID:12554641]

3. Ju, M; Wray, D. (2002) Molecular identification and characterisation of the human eag2 potassium channel. FEBS Lett., 524 (1-3): 204-10. [PMID:12135768]

4. Ludwig, J; Owen, D; Pongs, O. (1997) Carboxy-terminal domain mediates assembly of the voltage-gated rat ether-à-go-go potassium channel. EMBO J., 16 (21): 6337-45. [PMID:9400421]

5. Ludwig, J; Weseloh, R; Karschin, C; Liu, Q; Netzer, R; Engeland, B; Stansfeld, C; Pongs, O. (2000) Cloning and functional expression of rat eag2, a new member of the ether-à-go-go family of potassium channels and comparison of its distribution with that of eag1. Mol. Cell. Neurosci., 16 (1): 59-70. [PMID:10882483]

6. Saganich, MJ; Vega-Saenz de Miera, E; Nadal, MS; Baker, H; Coetzee, WA; Rudy, B. (1999) Cloning of components of a novel subthreshold-activating K(+) channel with a unique pattern of expression in the cerebral cortex. J. Neurosci., 19 (24): 10789-802. [PMID:10594062]

To cite this database page, please use the following:

K. George Chandy, Stephan Grissmer, George A. Gutman, Michel Lazdunski, David Mckinnon, Luis A. Pardo, Gail A. Robertson, Bernardo Rudy, Michael C. Sanguinetti, Walter Stühmer, Xiaoliang Wang.
Voltage-gated potassium channels: Kv10.2. Last modified on 01/07/2010. Accessed on 24/05/2013. IUPHAR database (IUPHAR-DB), http://www.iuphar-db.org/DATABASE/ObjectDisplayForward?objectId=571.


Contact us | Print | Back to top | Help