Nomenclature: Kv1.2

Family: Voltage-gated potassium channels

Annotation status:  image of a green circle Annotated and expert reviewed. Please contact us if you can help with updates. 

Contents

Gene and Protein Information
Species TM P Loops AA Chromosomal Location Gene Symbol Gene Name Reference
Human 6 1 499 1p13 KCNA2 potassium voltage-gated channel, shaker-related subfamily, member 2 6,16
Mouse 6 1 499 3 F2.3 Kcna2 potassium voltage-gated channel, shaker-related subfamily, member 2
Rat 6 1 499 2q34 Kcna2 potassium voltage-gated channel, shaker-related subfamily, member 2
Previous and Unofficial Names
HuK (IV)
MK2
BK2
RCK5
RAK
BGK5
XSha2
NGK1
HBK51
Kv1.2
HK4
Potassium (K+) channel protein alpha 2 voltage dependent
Potassium (K+) channel protein alpha 2, voltage dependent
Potassium voltage gated channel shaker related subfamily member 2
RBK2
potassium voltage gated channel, shaker related subfamily, member 2
potassium voltage-gated channel subfamily A member 2
potassium voltage-gated channel, shaker-related subfamily, member 2
voltage-gated potassium channel subunit Kv1.2
Mk-2
Kca1-2
Akr6a4
Database Links
ChEMBL Target
Ensembl Gene
Entrez Gene
GeneCards
GenitoUrinary Development Molecular Anatomy Project
HomoloGene
Human Protein Reference Database
InterPro
KEGG Gene
OMIM
PharmGKB Gene
PhosphoSitePlus
Protein Ontology (PRO)
RefSeq Nucleotide
RefSeq Protein
TreeFam
UniGene Hs.
UniProtKB
Wikipedia
Associated Proteins
Heteromeric Pore-forming Subunits
Name References
Kvβ1 22,24,26
Kvβ2 23-24,27
Auxiliary Subunits
Name References
Not determined
Other Associated Proteins
Name References
PSD95 9
RhoA 2
Caspr2 18
SAP97 32
SNAP95 32
Functional Characteristics
KV
Ion Selectivity and Conductance
Species:  Mouse
Rank order:  K+ [14.0 - 18.0 pS]
References:  7
Species:  Mouse
Macroscopic current rectification:  Ik: Delayed Rectifier K+ current
References:  7
Voltage Dependence
  V0.5 (mV)  τ (msec)  Reference  Cell type  Species 
Activation  5.0 – 27.0 6.0 7,21 B82 mouse fibroblasts Mouse
Inactivation  -15.0 - 7
Associated subunits (Human)
Kv β1 and Kv β2
Channel Blockers
Key to terms and symbols View all chemical structures Click column headers to sort
Ligand Sp. Action Affinity Units Concentration range (M) Holding voltage (mV) Reference
hongotoxin-1 Rn - 12.6 – 13.0 pKd - - 12
pKd 12.6 – 13.0 [12]
noxiustoxin Rn - 8.7 pKd - - 7
pKd 8.7 [7]
charybdotoxin Rn - 7.9 pKd - - 7
pKd 7.9 [7]
dendrotoxin-1 Rn - 7.8 pKd - - 7
pKd 7.8 [7]
α-KTx 13.2 Rn - 7.0 pKd - - 5
pKd 7.0 [5]
pandinotoxin-K α Rn - 10.5 pIC50 - - 8,25
pIC50 10.5 [8,25]
BgK Rn - 7.6 pIC50 - - 4
pIC50 7.6 [4]
mast cell degranulating peptide Rn - 6.4 pIC50 - - 8
pIC50 6.4 [8]
Channel Blocker Comments
Anandamide pKd= 5.6 [19], capsaicin pKd= 4.3, resiniferatoxin pKd= 4.5, flecanide pKd= 3.7, nifedipine pKd=4.7, diltazem pKd=3.7, tetraethylammonium pKd=0.3, 4-aminopyridine pKd=3.2 [7-8,35].
Tissue Distribution
Schwann cells and sciatic nerve
Species:  Mouse
Technique:  Oligonucleic probes
References:  1,30
Brain
Species:  Mouse
Technique:  In situ hybridisation
References:  3
Spinal cord
Species:  Mouse
Technique:  Immunohistochemistry, western blotting
References:  28
Retina
Species:  Mouse
Technique:  RT-PCR
References:  10-11
Skeletal and cardiac muscles
Species:  Rat
Technique:  Northern Blot
References:  14
Retina, islet
Species:  Rat
Technique:  RT-PCR
References:  8
Brain (pons, medulla, cerebellum, inferior colliculus > hippocampus, thalamus, cerebral cortex, superior colliculus > midbrain, corpus striatum, olfactory bulb)(neurons associated with mechanoreception and proprioception)
Species:  Rat
Technique:  RT-PCR
References:  15,31
Functional Assays
Molecular clone and voltage clamp
Species:  Rat
Tissue:  Xenopus leavis Oocytes
Response measured:  Delayed rectifier potassium currents
References:  7,31
Physiological Functions
Maintaining membrane potential, modulating electrical excitability in neurons and muscle.
Species:  Rat
Tissue:  CNS and muscle
References:  7-8,14,20,28-29,31,36
Phenotypes, Alleles and Disease Models Mouse data from MGI

Show »

Allele Composition & genetic background Accession Phenotype Id Phenotype Reference
Kcna2tm1Tem Kcna2tm1Tem/Kcna2tm1Tem
C3Fe.129S7-Kcna2
MGI:96659  MP:0005402 abnormal action potential PMID: 17634333 
Kcna2tm1Tem Kcna2tm1Tem/Kcna2tm1Tem
involves: 129S7/SvEvBrd
MGI:96659  MP:0004994 abnormal brain wave pattern PMID: 17925011 
Kcna2tm1Tem Kcna2tm1Tem/Kcna2tm1Tem
C3Fe.129S7-Kcna2
MGI:96659  MP:0003484 abnormal channel response PMID: 17634333 
Kcna2tm1Tem Kcna2tm1Tem/Kcna2tm1Tem
C3Fe.129S7-Kcna2
MGI:96659  MP:0002272 abnormal nervous system electrophysiology PMID: 17634333 
Kcna2tm1Tem Kcna2tm1Tem/Kcna2tm1Tem
involves: 129S7/SvEvBrd
MGI:96659  MP:0001501 abnormal sleep pattern PMID: 17925011 
Kcna2tm1Tem Kcna2tm1Tem/Kcna2tm1Tem
C3Fe.129S7-Kcna2
MGI:96659  MP:0001957 apnea PMID: 17634333 
Kcna2tm1Tem Kcna2tm1Tem/Kcna2tm1Tem
involves: 129S7/SvEvBrd
MGI:96659  MP:0001265 decreased body size PMID: 17925011 
Kcna2tm1Tem Kcna2tm1Tem/Kcna2tm1Tem
C3Fe.129S7-Kcna2
MGI:96659  MP:0001262 decreased body weight PMID: 17634333 
Kcna2+|Kcna2tm1Tem Kcna2tm1Tem/Kcna2+
C3Fe.129S7-Kcna2
MGI:96659  MP:0002578 impaired ability to fire action potentials PMID: 17634333 
Kcna2tm1Tem Kcna2tm1Tem/Kcna2tm1Tem
C3Fe.129S7-Kcna2
MGI:96659  MP:0002578 impaired ability to fire action potentials PMID: 17634333 
Kcna2+|Kcna2tm1Tem Kcna2tm1Tem/Kcna2+
C3Fe.129S7-Kcna2
MGI:96659  MP:0002906 increased susceptibility to pharmacologically induced seizures PMID: 17634333 
Kcna2tm1Tem Kcna2tm1Tem/Kcna2tm1Tem
C3Fe.129S7-Kcna2
MGI:96659  MP:0002906 increased susceptibility to pharmacologically induced seizures PMID: 17634333 
Kcna2tm1Tem Kcna2tm1Tem/Kcna2tm1Tem
involves: 129S7/SvEvBrd * C57BL/6
MGI:96659  MP:0002082 postnatal lethality PMID: 17634333 
Kcna2tm1Tem Kcna2tm1Tem/Kcna2tm1Tem
C3Fe.129S7-Kcna2
MGI:96659  MP:0002082 postnatal lethality PMID: 17634333 
Kcna2tm1Tem Kcna2tm1Tem/Kcna2tm1Tem
involves: 129S7/SvEvBrd
MGI:96659  MP:0002082 postnatal lethality PMID: 17925011 
Kcna2tm1Tem Kcna2tm1Tem/Kcna2tm1Tem
involves: 129S7/SvEvBrd
MGI:96659  MP:0002064 seizures PMID: 17925011 
Kcna2tm1Tem Kcna2tm1Tem/Kcna2tm1Tem
involves: 129S7/SvEvBrd * C57BL/6
MGI:96659  MP:0002826 tonic seizures PMID: 17634333 
Kcna2tm1Tem Kcna2tm1Tem/Kcna2tm1Tem
C3Fe.129S7-Kcna2
MGI:96659  MP:0002826 tonic seizures PMID: 17634333 
Gene Expression and Pathophysiology
Decrease
Tissue or cell type:  Heart, hippocampus
Pathophysiology:  Seizure activity caused a reduction of Kv1.2 and Kv4.2 mRNAs in the dentate granule cells of the hippocampus.
Acute and chronic electroconvulsive shock reduces Kv1.2 and Kv4.2 mRNA abundance.
Hypoxia inhibits gene expression of Kv1.2 in pulmonary artery smooth muscle cells.
Kv1.2 mRNA downregulated in rat heart with right ventricular hypertrophy.
Species:  Rat
Technique: 
References:  13,17,33-34
General Comments
Kv1.2 can coassemble with other Kv1 family members in heteromultimers but not with members of other Kv families. It is unique in that it has an intronless coding region.

REFERENCES

1. Black JA, Waxman SG, Smith KJ. (2006) Remyelination of dorsal column axons by endogenous Schwann cells restores the normal pattern of Nav1.6 and Kv1.2 at nodes of Ranvier. Brain129 (Pt 5): 1319-29. [PMID:16537565]

2. Cachero TG, Morielli AD, Peralta EG. (1998) The small GTP-binding protein RhoA regulates a delayed rectifier potassium channel. Cell93 (6): 1077-85. [PMID:9635436]

3. Chandy KG, Williams CB, Spencer RH, Aguilar BA, Ghanshani S, Tempel BL, Gutman GA. (1990) A family of three mouse potassium channel genes with intronless coding regions. Science247 (4945): 973-5. [PMID:2305265]

4. Cotton J, Crest M, Bouet F, Alessandri N, Gola M, Forest E, Karlsson E, Castañeda O, Harvey AL, Vita C, Ménez A. (1997) A potassium-channel toxin from the sea anemone Bunodosoma granulifera, an inhibitor for Kv1 channels. Revision of the amino acid sequence, disulfide-bridge assignment, chemical synthesis, and biological activity. Eur. J. Biochem.244 (1): 192-202. [PMID:9063464]

5. Dudina EE, Korolkova YV, Bocharova NE, Koshelev SG, Egorov TA, Huys I, Tytgat J, Grishin EV. (2001) OsK2, a new selective inhibitor of Kv1.2 potassium channels purified from the venom of the scorpion Orthochirus scrobiculosus. Biochem. Biophys. Res. Commun.286 (5): 841-7. [PMID:11527374]

6. Grissmer S, Dethlefs B, Wasmuth JJ, Goldin AL, Gutman GA, Cahalan MD, Chandy KG. (1990) Expression and chromosomal localization of a lymphocyte K+ channel gene. Proc. Natl. Acad. Sci. U.S.A.87 (23): 9411-5. [PMID:2251283]

7. Grissmer S, Nguyen AN, Aiyar J, Hanson DC, Mather RJ, Gutman GA, Karmilowicz MJ, Auperin DD, Chandy KG. (1994) Pharmacological characterization of five cloned voltage-gated K+ channels, types Kv1.1, 1.2, 1.3, 1.5, and 3.1, stably expressed in mammalian cell lines. Mol. Pharmacol.45 (6): 1227-34. [PMID:7517498]

8. Gutman GA, Chandy KG, Grissmer S, Lazdunski M, McKinnon D, Pardo LA, Robertson GA, Rudy B, Sanguinetti MC, Stühmer W, Wang X. (2005) International Union of Pharmacology. LIII. Nomenclature and molecular relationships of voltage-gated potassium channels. Pharmacol. Rev.57 (4): 473-508. [PMID:16382104]

9. Kim E, Niethammer M, Rothschild A, Jan YN, Sheng M. (1995) Clustering of Shaker-type K+ channels by interaction with a family of membrane-associated guanylate kinases. Nature378 (6552): 85-8. [PMID:7477295]

10. Klumpp DJ, Song EJ, Ito S, Sheng MH, Jan LY, Pinto LH. (1995) The Shaker-like potassium channels of the mouse rod bipolar cell and their contributions to the membrane current. J. Neurosci.15 (7 Pt 1): 5004-13. [PMID:7623129]

11. Klumpp DJ, Song EJ, Pinto LH. (1995) Identification and localization of K+ channels in the mouse retina. Vis. Neurosci.12 (6): 1177-90. [PMID:8962835]

12. Koschak A, Bugianesi RM, Mitterdorfer J, Kaczorowski GJ, Garcia ML, Knaus HG. (1998) Subunit composition of brain voltage-gated potassium channels determined by hongotoxin-1, a novel peptide derived from Centruroides limbatus venom. J. Biol. Chem.273 (5): 2639-44. [PMID:9446567]

13. Lee JK, Nishiyama A, Kambe F, Seo H, Takeuchi S, Kamiya K, Kodama I, Toyama J. (1999) Downregulation of voltage-gated K(+) channels in rat heart with right ventricular hypertrophy. Am. J. Physiol.277 (5 Pt 2): H1725-31. [PMID:10564125]

14. Matsubara H, Liman ER, Hess P, Koren G. (1991) Pretranslational mechanisms determine the type of potassium channels expressed in the rat skeletal and cardiac muscles. J. Biol. Chem.266 (20): 13324-8. [PMID:1712780]

15. McKinnon D. (1989) Isolation of a cDNA clone coding for a putative second potassium channel indicates the existence of a gene family. J. Biol. Chem.264 (14): 8230-6. [PMID:2722779]

16. Minor DL, Lin YF, Mobley BC, Avelar A, Jan YN, Jan LY, Berger JM. (2000) The polar T1 interface is linked to conformational changes that open the voltage-gated potassium channel. Cell102 (5): 657-70. [PMID:11007484]

17. Pei Q, Burnet PW, Grahame-Smith DG, Zetterström TS. (1997) Differential effects of acute and chronic electroconvulsive shock on the abundance of messenger RNAs for voltage-dependent potassium channel subunits in the rat brain. Neuroscience78 (2): 343-50. [PMID:9145791]

18. Poliak S, Gollan L, Martinez R, Custer A, Einheber S, Salzer JL, Trimmer JS, Shrager P, Peles E. (1999) Caspr2, a new member of the neurexin superfamily, is localized at the juxtaparanodes of myelinated axons and associates with K+ channels. Neuron24 (4): 1037-47. [PMID:10624965]

19. Poling JS, Rogawski MA, Salem N, Vicini S. (1996) Anandamide, an endogenous cannabinoid, inhibits Shaker-related voltage-gated K+ channels. Neuropharmacology35 (7): 983-91. [PMID:8938728]

20. Ramashwami M Gautam M Kamb AA Rudy B Tanouye MA Mathew MK. (1990) Human Potassium Channel Genes: Molecular CLoning and Functional Expression. Molecular Cell Neurosciencevolume (1): 214 - 223.

21. Ramashwami M Gautam M Kamb AA Rudy B Tanouye MA Mathew MK. (1990) Human Potassium Channel Genes: Molecular CLoning and Functional Expression. Molecular Cell Neurosciencevolume (1): 214 - 223.

22. Rettig J, Heinemann SH, Wunder F, Lorra C, Parcej DN, Dolly JO, Pongs O. (1994) Inactivation properties of voltage-gated K+ channels altered by presence of beta-subunit. Nature369 (6478): 289-94. [PMID:8183366]

23. Rhodes KJ, Keilbaugh SA, Barrezueta NX, Lopez KL, Trimmer JS. (1995) Association and colocalization of K+ channel alpha- and beta-subunit polypeptides in rat brain. J. Neurosci.15 (7 Pt 2): 5360-71. [PMID:7623158]

24. Rhodes KJ, Strassle BW, Monaghan MM, Bekele-Arcuri Z, Matos MF, Trimmer JS. (1997) Association and colocalization of the Kvbeta1 and Kvbeta2 beta-subunits with Kv1 alpha-subunits in mammalian brain K+ channel complexes. J. Neurosci.17 (21): 8246-58. [PMID:9334400]

25. Rogowski RS, Collins JH, O'Neill TJ, Gustafson TA, Werkman TR, Rogawski MA, Tenenholz TC, Weber DJ, Blaustein MP. (1996) Three new toxins from the scorpion Pandinus imperator selectively block certain voltage-gated K+ channels. Mol. Pharmacol.50 (5): 1167-77. [PMID:8913348]

26. Scott VE, Rettig J, Parcej DN, Keen JN, Findlay JB, Pongs O, Dolly JO. (1994) Primary structure of a beta subunit of alpha-dendrotoxin-sensitive K+ channels from bovine brain. Proc. Natl. Acad. Sci. U.S.A.91 (5): 1637-41. [PMID:8127858]

27. Shi G, Nakahira K, Hammond S, Rhodes KJ, Schechter LE, Trimmer JS. (1996) Beta subunits promote K+ channel surface expression through effects early in biosynthesis. Neuron16 (4): 843-52. [PMID:8608002]

28. Sinha K, Karimi-Abdolrezaee S, Velumian AA, Fehlings MG. (2006) Functional changes in genetically dysmyelinated spinal cord axons of shiverer mice: role of juxtaparanodal Kv1 family K+ channels. J. Neurophysiol.95 (3): 1683-95. [PMID:16319208]

29. Sobko A, Peretz A, Attali B. (1998) Constitutive activation of delayed-rectifier potassium channels by a src family tyrosine kinase in Schwann cells. EMBO J.17 (16): 4723-34. [PMID:9707431]

30. Sobko A, Peretz A, Shirihai O, Etkin S, Cherepanova V, Dagan D, Attali B. (1998) Heteromultimeric delayed-rectifier K+ channels in schwann cells: developmental expression and role in cell proliferation. J. Neurosci.18 (24): 10398-408. [PMID:9852577]

31. Stühmer W, Ruppersberg JP, Schröter KH, Sakmann B, Stocker M, Giese KP, Perschke A, Baumann A, Pongs O. (1989) Molecular basis of functional diversity of voltage-gated potassium channels in mammalian brain. EMBO J.8 (11): 3235-44. [PMID:2555158]

32. Tiffany AM, Manganas LN, Kim E, Hsueh YP, Sheng M, Trimmer JS. (2000) PSD-95 and SAP97 exhibit distinct mechanisms for regulating K(+) channel surface expression and clustering. J. Cell Biol.148 (1): 147-58. [PMID:10629225]

33. Tsaur ML, Sheng M, Lowenstein DH, Jan YN, Jan LY. (1992) Differential expression of K+ channel mRNAs in the rat brain and down-regulation in the hippocampus following seizures. Neuron8 (6): 1055-67. [PMID:1610565]

34. Wang J, Juhaszova M, Rubin LJ, Yuan XJ. (1997) Hypoxia inhibits gene expression of voltage-gated K+ channel alpha subunits in pulmonary artery smooth muscle cells. J. Clin. Invest.100 (9): 2347-53. [PMID:9410914]

35. Wulff H, Rauer H, Düring T, Hanselmann C, Ruff K, Wrisch A, Grissmer S, Hänsel W. (1998) Alkoxypsoralens, novel nonpeptide blockers of Shaker-type K+ channels: synthesis and photoreactivity. J. Med. Chem.41 (23): 4542-9. [PMID:9804693]

36. Yazulla S, Studholme KM. (1998) Differential distribution of Shaker-like and Shab-like K+-channel subunits in goldfish retina and retinal bipolar cells. J. Comp. Neurol.396 (1): 131-40. [PMID:9623892]

To cite this database page, please use the following:

K. George Chandy, Stephan Grissmer, George A. Gutman, Michel Lazdunski, David Mckinnon, Luis A. Pardo, Gail A. Robertson, Bernardo Rudy, Michael C. Sanguinetti, Walter Stühmer, Xiaoliang Wang.
Voltage-gated potassium channels: Kv1.2. Last modified on 31/01/2014. Accessed on 27/08/2014. IUPHAR database (IUPHAR-DB), http://www.iuphar-db.org/DATABASE/ObjectDisplayForward?objectId=539.

Contact us | Print | Back to top | Help
Copyright © 2014 IUPHAR