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TRPP2

Family: Transient Receptor Potential channels

Contents:
Gene and Protein Information
Previous and Unofficial Names
Database Links
Associated Proteins
Ion Selectivity and Conductance
Activators
Pore Blockers
Tissue Distribution
Functional Assays
Physiological Functions
Clinically-Relevant Mutations and Pathophysiology
References
Gene and Protein Information
Species TM P Loops AA Chromosomal Location Gene Symbol Gene Name Reference
Human 6 1 805 10q24.31 PKD2L1 polycystic kidney disease 2-like 1 2,7-8
Mouse 6 1 760 19 C3 Pkd2l1 polycystic kidney disease 2-like 1 6-7
Rat 6 1 676 1q56 Pkd2l1 polycystic kidney disease 2-like 1
Previous and Unofficial Names
PCL (polycystin-like)
PKDL
PKD2L
PKD2L1
PKDL2
polycystic kidney disease 2-like 1 protein
PCL
TRPP3
transient receptor potential cation channel, subfamily P, member 3
LOC293937
Pkd2l1_predicted
polycystic kidney disease 2-like 1
polycystic kidney disease 2-like 1 (predicted)
polycystin-L
BC046386
Database Links
Ensembl ENSG00000107593 (Hs), ENSMUSG00000037578 (Mm), ENSRNOG00000012591 (Rn)
Entrez Gene 9033 (Hs), 329064 (Mm), 293937 (Rn)
GeneCards PKD2L1 (Hs)
GenitoUrinary Development Molecular Anatomy Project Pkd2l1 (Mm)
HomoloGene 22946 (Hs)
Human Protein Reference Database 16063 (Hs)
InterPro Q9P0L9 (Hs)
KEGG Gene hsa:9033 (Hs), mmu:329064 (Mm), rno:293937 (Rn)
OMIM 604532 (Hs)
PharmGKB Gene PA33344 (Hs)
PhosphoSitePlus Q9P0L9 (Hs)
Protein Ontology (PRO) PRO:000012774 (Hs)
RefSeq Nucleotide NM_016112 (Hs), NM_181422 (Mm), NM_001106352 (Rn)
RefSeq Protein NP_057196 (Hs), NP_852087 (Mm), NP_001099822 (Rn)
TreeFam ENSG00000107593 (Hs), ENSMUSG00000037578 (Mm), ENSRNOG00000012591 (Rn)
UniGene Hs. 159241 (Hs)
UniProt Q9P0L9 (Hs)
Wikipedia TRPP2
Transient Receptor Potential channels
Search for 3D structures on the PDB
Search by keyword: Transient Receptor Potential channels TRPP2 Search by accession number: Q9P0L9
Associated Proteins
Heteromeric Pore-forming Subunits
Name References
Not determined
Auxiliary Subunits
Name References
PKD1L3 4
Other Associated Proteins
Name References
Not determined
Ion Selectivity and Conductance
Species:  Human
Rank order:  Na+ [137.0 pS] > Ca2+ [120.0 - 135.0 pS] = Sr2+ = Ba2+
References:  1
Activators (Human)
Low constitutive activity, enhanced by membrane depolarization; changes in cell volume affect voltage-dependent gating (increased channel opening probability with cell swelling)
Activators
Key to terms and symbols View all chemical structures Click column headers to sort
Ligand Sp. Affinity Units Concentration range (M) Holding voltage (mV) Reference
malic acid Hs - - - Physiological 4
Cao2+ Hs - - 5x10-3 -150.0 – 100.0 1
Cai2+ Hs - - 1x10-6 -60.0 – -50.0 1
HCl Hs 1.89 pEC50 1.1x10-2 - 1.5x10-2 Physiological 4
citric acid Hs 1.7 pEC50 1.5x10-2 - 3x10-2 Physiological 4
Activator Comments
Chen et al [1] expressed human TRPP2 in Xenopus oocytes and proposed increased currents on increasing external or (inferred) internal Ca2+ levels and noted a decrease in channel activity on lowering external pH (7.5>6). TRPP2 and PKD1L3 are coexpressed in sour taste receptor cells of the tongue. Coexpression of the PDK1L3 and TRPP2 is necessary for functional cell surface expression, presumably as a heteromeric complex, where they are activated by acid pH [4].
Pore Blockers
Key to terms and symbols View all chemical structures Click column headers to sort
Ligand Sp. Affinity Units Concentration range (M) Holding voltage (mV) Reference
flufenamic acid Hs - - 5x10-4 -50.0 1
Gd3+ Hs - - 1x10-4 -50.0 1
La3+ Hs - - 1x10-4 -50.0 1
Pore Blocker Comments
The block refers to the inhibition of the presumed current carried by human TRPP2 in Xenopus laevis oocytes.

Explore drug-target interactions for this set of compounds using iPHACE

Tissue Distribution
Adult: Heart, skeletal muscle, brain, spleen, testis, retina
Fetal: Widely expressed, higher expression than adult.
Species:  Human
Technique:  Northern Blot
References:  7-8
"Taste tissue", testis > wide expression at low levels.
Species:  Mouse
Technique:  RT-PCR
References:  4
Sour taste receptors of the tongue, circumvallate and foliate papilla, cells surrounding the central canal of the spinal cord.
Species:  Mouse
Technique:  Immunohistochemistry
References:  3-4
Circumvallate, foliate and fungiform papillae, palate, cells surrounding the central canal of the spinal cord, hypothalamus.
Species:  Mouse
Technique:  In situ hybridisation
References:  3-5
Functional Assays
Imaging of calcium influx into cells stimulated by citric acid or HCL.
Species:  Mouse
Tissue:  HEK 293 cells transiently expressing TRPP2 and PKD1L3
Response measured:  Intracellular Ca2+
References:  4
Patch-clamp recordings of the response of TRPP2-positive cells to changes in pH.
Species:  Mouse
Tissue:  Spinal cord slice preparation
Response measured:  Action potential frequency
References:  3
Patch clamp measurement of non-selective cation currents at rest and stimulated by Ca2+.
Species:  Human
Tissue:  expressed in Xenopus oocytes
Response measured:  Current
References:  1
Physiological Functions
Acid sensing in sour taste.
Species:  Mouse
Tissue:  Tongue, palate.
References:  3-4
Acid sensing in cerebrospinal fluid.
Species:  Mouse
Tissue:  Spinal cord cells adjacent to the central canal.
References:  3
Clinically-Relevant Mutations and Pathophysiology Comments
TRPP2 is deleted in the Krd mouse. This mouse has defects in structures of the kidney, retina and optic disc, however the Krd deletion is a 7cM transgene-induce deletion on chromosome 19 that includes the Pax2 locus [7].

REFERENCES

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1. Chen, XZ; Vassilev, PM; Basora, N; Peng, JB; Nomura, H; Segal, Y; Brown, EM; Reeders, ST; Hediger, MA; Zhou, J. (1999) Polycystin-L is a calcium-regulated cation channel permeable to calcium ions. Nature, 401 (6751): 383-6. [PMID:10517637]

2. Guo, L; Chen, M; Basora, N; Zhou, J. (2000) The human polycystic kidney disease 2-like (PKDL) gene: exon/intron structure and evidence for a novel splicing mechanism. Mamm. Genome, 11 (1): 46-50. [PMID:10602992]

3. Huang, AL; Chen, X; Hoon, MA; Chandrashekar, J; Guo, W; Tränkner, D; Ryba, NJ; Zuker, CS. (2006) The cells and logic for mammalian sour taste detection. Nature, 442 (7105): 934-8. [PMID:16929298]

4. Ishimaru, Y; Inada, H; Kubota, M; Zhuang, H; Tominaga, M; Matsunami, H. (2006) Transient receptor potential family members PKD1L3 and PKD2L1 form a candidate sour taste receptor. Proc. Natl. Acad. Sci. U.S.A., 103 (33): 12569-74. [PMID:16891422]

5. LopezJimenez, ND; Cavenagh, MM; Sainz, E; Cruz-Ithier, MA; Battey, JF; Sullivan, SL. (2006) Two members of the TRPP family of ion channels, Pkd1l3 and Pkd2l1, are co-expressed in a subset of taste receptor cells. J. Neurochem., 98 (1): 68-77. [PMID:16805797]

6. Murakami, M; Ohba, T; Xu, F; Shida, S; Satoh, E; Ono, K; Miyoshi, I; Watanabe, H; Ito, H; Iijima, T. (2005) Genomic organization and functional analysis of murine PKD2L1. J. Biol. Chem., 280 (7): 5626-35. [PMID:15548533]

7. Nomura, H; Turco, AE; Pei, Y; Kalaydjieva, L; Schiavello, T; Weremowicz, S; Ji, W; Morton, CC; Meisler, M; Reeders, ST; Zhou, J. (1998) Identification of PKDL, a novel polycystic kidney disease 2-like gene whose murine homologue is deleted in mice with kidney and retinal defects. J. Biol. Chem., 273 (40): 25967-73. [PMID:9748274]

8. Wu, G; Hayashi, T; Park, JH; Dixit, M; Reynolds, DM; Li, L; Maeda, Y; Cai, Y; Coca-Prados, M; Somlo, S. (1998) Identification of PKD2L, a human PKD2-related gene: tissue-specific expression and mapping to chromosome 10q25. Genomics, 54 (3): 564-8. [PMID:9878261]

To cite this database page, please use the following:

David E. Clapham, Bernd Nilius, Grzegorz Owsianik.
Transient Receptor Potential channels: TRPP2. Last modified on 02/05/2012. Accessed on 21/05/2013. IUPHAR database (IUPHAR-DB), http://www.iuphar-db.org/DATABASE/ObjectDisplayForward?objectId=505.


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