image of a green circle Annotated and expert reviewed. Please contact us if you can help with updates. 

Kir5.1

Family: Inwardly rectifying potassium channels

Contents:
Gene and Protein Information
Previous and Unofficial Names
Database Links
Associated Proteins
Ion Selectivity and Conductance
Gating Inhibitors
Pore Blockers
Tissue Distribution
Physiological Functions
References
Gene and Protein Information
Species TM P Loops AA Chromosomal Location Gene Symbol Gene Name Reference
Human 2 1 418 17q23.1-24.2 KCNJ16 potassium inwardly-rectifying channel, subfamily J, member 16
Mouse 2 1 419 11 E2 Kcnj16 potassium inwardly-rectifying channel, subfamily J, member 16 7
Rat 2 1 419 10q32.1 Kcnj16 potassium inwardly-rectifying channel, subfamily J, member 16 1,12
Previous and Unofficial Names
BIR9
Kir5.1
inward rectifier K(+) channel Kir5.1
inward rectifier potassium channel 16
potassium channel, inwardly rectifying subfamily J member 16
potassium inwardly-rectifying channel, subfamily J, member 16
6430410F18Rik
AI132396
Database Links
Ensembl ENSG00000153822 (Hs), ENSMUSG00000051497 (Mm), ENSRNOG00000004713 (Rn)
Entrez Gene 3773 (Hs), 16517 (Mm), 29719 (Rn)
GeneCards KCNJ16 (Hs)
GenitoUrinary Development Molecular Anatomy Project Kcnj16 (Mm)
HomoloGene 23112 (Hs)
Human Protein Reference Database 16146 (Hs)
InterPro Q9NPI9 (Hs), Q9Z307 (Mm), P52191 (Rn)
KEGG Gene hsa:3773 (Hs), mmu:16517 (Mm), rno:29719 (Rn)
OMIM 605722 (Hs)
PharmGKB Gene PA30047 (Hs)
PhosphoSitePlus Q9NPI9 (Hs), Q9Z307 (Mm), P52191 (Rn)
Protein Ontology (PRO) PRO:000002055 (Hs)
RefSeq Nucleotide NM_018658 (Hs), NM_010604 (Mm), NM_053314 (Rn)
RefSeq Protein NP_061128 (Hs), NP_034734 (Mm), NP_445766 (Rn)
TreeFam ENSG00000153822 (Hs), ENSMUSG00000051497 (Mm), ENSRNOG00000004713 (Rn)
UniGene Hs. 463985 (Hs)
UniProt Q9NPI9 (Hs), Q9Z307 (Mm), P52191 (Rn)
Wikipedia Kir5.1
Potassium channels
Search for 3D structures on the PDB
Search by keyword: Inwardly rectifying potassium channels Kir5.1 Search by accession number: P52191, Q9Z307, Q9NPI9
Associated Proteins
Heteromeric Pore-forming Subunits
Name References
Kir4.2 8
Kir4.1 8
Auxiliary Subunits
Name References
Not determined
Other Associated Proteins
Name References
PSD-95 10
Ion Selectivity and Conductance
Species:  Rat
Rank order:  K+
References:  10
Ion Selectivity and Conductance Comments
Kir5.1 only forms a functional homomeric channel when expressed with PSD-95 [10].

Functional channels are also formed by heteromers of Kir5.1 with
· Kir4.1 (K+,42.7-49.6pS, [9]
· Kir4.2 (K+, 54.2pS, [8])
Gating Inhibitor Comments
PKA phosphorylation inhibits tha activation of the Kir5.1/PSD-95 channel [10].
Pore Blockers
Key to terms and symbols Click column headers to sort
Ligand Sp. Affinity Units Concentration range (M) Holding voltage (mV) Reference
Cs+ Rn - - - Physiological 1
Ba2+ Rn - - 3x10-3 -100.0 1,10
Pore Blocker Comments
Kir5.1, when forming heteromers with Kir4.1 or Kir4.2, modulates their sensitivity to intracellular pH:
· Kir4.1/5.1 heteromer (pEC50=6.8-7.35, [8,11-12]).
· Kir4.2/5.1 heteromer (pEC50=7.64, [8]).
Tissue Distribution
Kidney (convoluted tubule cells), pancreas (acinar and ductal cells), thyroid gland.
Species:  Human
Technique:  In situ hybridisation
References:  6
Astrocytes (forebrain, olfactory bulb).
Species:  Mouse
Technique:  Immunohistochemistry
References:  3
Retina (Müller cells, GABAergic amacrine cells).
Species:  Rat
Technique:  Immunohistochemistry
References:  5
Cochlea (spiral ligament of the lateral wall).
Species:  Rat
Technique:  Immunohistochemistry
References:  4
Brainstem nuclei (mesencephalic trigeminal, hypoglossal nucleus, facial, lateral reticular, para-ambiguus, paragigantocellularis, pontine), locus coeruleus, superior and inferior colliculi.
Species:  Rat
Technique:  In situ hybridisation
References:  2,13
Spleen, adrenal gland, liver, testis
Species:  Rat
Technique:  Northern Blot
References:  1
Functional Assays
Not Determined
Physiological Functions
pH sensing.
Species:  Rat
Tissue:  Kidney
References:  12

REFERENCES

Show »

1. Bond, CT; Pessia, M; Xia, XM; Lagrutta, A; Kavanaugh, MP; Adelman, JP. (1994) Cloning and expression of a family of inward rectifier potassium channels. Recept. Channels, 2 (3): 183-91. [PMID:7874445]

2. Derst, C; Karschin, C; Wischmeyer, E; Hirsch, JR; Preisig-Müller, R; Rajan, S; Engel, H; Grzeschik, K; Daut, J; Karschin, A. (2001) Genetic and functional linkage of Kir5.1 and Kir2.1 channel subunits. FEBS Lett., 491 (3): 305-11. [PMID:11240146]

3. Hibino, H; Fujita, A; Iwai, K; Yamada, M; Kurachi, Y. (2004) Differential assembly of inwardly rectifying K+ channel subunits, Kir4.1 and Kir5.1, in brain astrocytes. J. Biol. Chem., 279 (42): 44065-73. [PMID:15310750]

4. Hibino, H; Higashi-Shingai, K; Fujita, A; Iwai, K; Ishii, M; Kurachi, Y. (2004) Expression of an inwardly rectifying K+ channel, Kir5.1, in specific types of fibrocytes in the cochlear lateral wall suggests its functional importance in the establishment of endocochlear potential. Eur. J. Neurosci., 19 (1): 76-84. [PMID:14750965]

5. Ishii, M; Fujita, A; Iwai, K; Kusaka, S; Higashi, K; Inanobe, A; Hibino, H; Kurachi, Y. (2003) Differential expression and distribution of Kir5.1 and Kir4.1 inwardly rectifying K+ channels in retina. Am. J. Physiol., Cell Physiol., 285 (2): C260-7. [PMID:12686518]

6. Liu, Y; McKenna, E; Figueroa, DJ; Blevins, R; Austin, CP; Bennett, PB; Swanson, R. (2000) The human inward rectifier K(+) channel subunit kir5.1 (KCNJ16) maps to chromosome 17q25 and is expressed in kidney and pancreas. Cytogenet. Cell Genet., 90 (1-2): 60-3. [PMID:11060447]

7. Mouri, T; Kittaka, N; Horio, Y; Copeland, NG; Gilbert, DJ; Jenkins, NA; Kurachi, Y. (1998) Assignment of mouse inwardly rectifying potassium channel Kcnj16 to the distal region of mouse chromosome 11. Genomics, 54 (1): 181-2. [PMID:9806850]

8. Pessia, M; Imbrici, P; D'Adamo, MC; Salvatore, L; Tucker, SJ. (2001) Differential pH sensitivity of Kir4.1 and Kir4.2 potassium channels and their modulation by heteropolymerisation with Kir5.1. J. Physiol. (Lond.), 532 (Pt 2): 359-67. [PMID:11306656]

9. Pessia, M; Tucker, SJ; Lee, K; Bond, CT; Adelman, JP. (1996) Subunit positional effects revealed by novel heteromeric inwardly rectifying K+ channels. EMBO J., 15 (12): 2980-7. [PMID:8670799]

10. Tanemoto, M; Fujita, A; Higashi, K; Kurachi, Y. (2002) PSD-95 mediates formation of a functional homomeric Kir5.1 channel in the brain. Neuron, 34 (3): 387-97. [PMID:11988170]

11. Tanemoto, M; Kittaka, N; Inanobe, A; Kurachi, Y. (2000) In vivo formation of a proton-sensitive K+ channel by heteromeric subunit assembly of Kir5.1 with Kir4.1. J. Physiol. (Lond.), 525 Pt 3: 587-92. [PMID:10856114]

12. Tucker, SJ; Imbrici, P; Salvatore, L; D'Adamo, MC; Pessia, M. (2000) pH dependence of the inwardly rectifying potassium channel, Kir5.1, and localization in renal tubular epithelia. J. Biol. Chem., 275 (22): 16404-7. [PMID:10764726]

13. Wu, J; Xu, H; Shen, W; Jiang, C. (2004) Expression and coexpression of CO2-sensitive Kir channels in brainstem neurons of rats. J. Membr. Biol., 197 (3): 179-91. [PMID:15042349]

To cite this database page, please use the following:

John P. Adelman, David E. Clapham, Hiroshi Hibino, Atsushi Inanobe, Lily Y. Jan, Andreas Karschin, Yoshihiro Kubo, Yoshihisa Kurachi, Michel Lazdunski, Takashi Miki, Colin G. Nichols, Wade L. Pearson, Susumu Seino, Carol A. Vandenberg.
Inwardly rectifying potassium channels: Kir5.1. Last modified on 01/07/2010. Accessed on 22/05/2013. IUPHAR database (IUPHAR-DB), http://www.iuphar-db.org/DATABASE/ObjectDisplayForward?objectId=440.


Contact us | Print | Back to top | Help