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CatSper3

Family: CatSper and Two-Pore channels

Contents:
Gene and Protein Information
Previous and Unofficial Names
Database Links
Associated Proteins
Tissue Distribution
Physiological Functions
Physiological Consequences of Altering Gene Expression
Phenotypes, Alleles and Disease Models
References
Gene and Protein Information
Species TM P Loops AA Chromosomal Location Gene Symbol Gene Name Reference
Human 6 1 398 5q31.1 CATSPER3 cation channel, sperm associated 3 6
Mouse 6 1 382 13 B2 Catsper3 cation channel, sperm associated 3 6
Rat 6 1 301 17p14 Catsper3 cation channel, sperm associated 3
Previous and Unofficial Names
CatSper4
Cation channel of sperm; 4
CACRC
Catsper3_predicted
LOC290989
cation channel sperm-associated protein 3
cation channel, sperm associated 3
cation channel, sperm associated 3 (predicted)
4921522D01Rik
Database Links
Ensembl ENSG00000152705 (Hs), ENSMUSG00000021499 (Mm)
Entrez Gene 347732 (Hs), 76856 (Mm), 290989 (Rn)
GeneCards CATSPER3 (Hs)
GenitoUrinary Development Molecular Anatomy Project Catsper3 (Mm)
HomoloGene 12658
Human Protein Reference Database 16447 (Hs)
InterPro Q86XQ3 (Hs), Q80W99 (Mm)
KEGG Gene hsa:347732 (Hs), mmu:76856 (Mm), rno:290989 (Rn)
OMIM 609120 (Hs)
PharmGKB Gene PA134911185 (Hs)
PhosphoSitePlus Q86XQ3 (Hs), Q80W99 (Mm)
Protein Ontology (PRO) PRO:000000671 (Hs)
RefSeq Nucleotide NM_178019 (Hs), NM_029772 (Mm), NM_001106101 (Rn)
RefSeq Protein NP_821138 (Hs), NP_084048 (Mm), NP_001099571 (Rn)
TreeFam ENSG00000152705 (Hs), ENSMUSG00000021499 (Mm)
UniGene Hs. 631804 (Hs)
UniProt Q86XQ3 (Hs), Q80W99 (Mm)
Wikipedia CatSper3
CatSper and Two-Pore channels
Search for 3D structures on the PDB
Search by keyword: CatSper and Two-Pore channels CatSper3 Search by accession number: Q80W99, Q86XQ3
Associated Proteins
Heteromeric Pore-forming Subunits
Name References
CatSper4 4
CatSper2 4
CatSper1 4
Auxiliary Subunits
Name References
Not determined
Other Associated Proteins
Name References
Not determined
Associated Protein Comments
CatSper1-4 appear to function only as a heterotetramer, producing a current known as ICatSper. All functional data for this channel can be found in CatSper1.
Tissue Distribution
Testis (principal piece of the tail of spermatozoa).
Species:  Mouse
Technique:  Immunocytochemistry
References:  2
Testis.
Species:  Mouse
Technique:  Northern Blot
References:  2,4
Mature spermatozoa.
Species:  Mouse
Technique:  In situ hybridisation
References:  2,4
Testis (expression observed from P20).
Species:  Mouse
Technique:  RT-PCR
References:  2
Physiological Functions
CatSper 1-4 together form a channel that is required for mouse sperm hyperactivation of motility, and consequently fertility.
Species:  Mouse
Tissue:  Spermatozoa
References:  1,3-6
Physiological Functions Comments
Sperm cells are slightly acidic internally (pH 6.8) in the vagina (~ pH 5) thus the pH dependence of ICatSper activation leaves it inactive at resting membrane potential. As the pH shifts to ~8 in the cervix and upper female reproductive tract the internal sperm cell pH also becomes alkaline (> pH 7.5) and ICatSper is activated, leading to hyperactivation of motility.
Physiological Consequences of Altering Gene Expression
Infertility as a consequence of loss of hyperactivation of motility.
Species:  Mouse
Tissue:  Spermatozoa
Technique:  CatSper3 knockout
References:  4
Phenotypes, Alleles and Disease Models Mouse data from MGI

Click here to show/hide data

Allele Composition & genetic background Accession Phenotype Id Phenotype Reference
Catsper3tm1Clph Catsper3tm1Clph/Catsper3tm1Clph
involves: 129S4/SvJae		 MGI:1924106  MP:0002674 abnormal sperm motility PMID: 17227845 
Catsper3tm1Wyan Catsper3tm1Wyan/Catsper3tm1Wyan
involves: 129S7/SvEvBrd * C57BL/6J		 MGI:1924106  MP:0002674 abnormal sperm motility PMID: 17344468 
Catsper3tm1Clph Catsper3tm1Clph/Catsper3tm1Clph
involves: 129S4/SvJae		 MGI:1924106  MP:0004543 abnormal sperm physiology PMID: 17227845 
Catsper3tm1Clph Catsper3tm1Clph/Catsper3tm1Clph
involves: 129S4/SvJae		 MGI:1924106  MP:0002675 asthenozoospermia PMID: 17227845 
Catsper3tm1Clph Catsper3tm1Clph/Catsper3tm1Clph
involves: 129S4/SvJae		 MGI:1924106  MP:0003666 impaired sperm capacitation PMID: 17227845 
Catsper3tm1Clph Catsper3tm1Clph/Catsper3tm1Clph
involves: 129S4/SvJae		 MGI:1924106  MP:0001925 male infertility PMID: 17227845 
Catsper3tm1Wyan Catsper3tm1Wyan/Catsper3tm1Wyan
involves: 129S7/SvEvBrd * C57BL/6J		 MGI:1924106  MP:0001925 male infertility PMID: 17344468 
Catsper3tm1Clph Catsper3tm1Clph/Catsper3tm1Clph
involves: 129S4/SvJae		 MGI:1924106  MP:0005578 teratozoospermia PMID: 17227845 

REFERENCES

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1. Carlson, AE; Westenbroek, RE; Quill, T; Ren, D; Clapham, DE; Hille, B; Garbers, DL; Babcock, DF. (2003) CatSper1 required for evoked Ca2+ entry and control of flagellar function in sperm. Proc. Natl. Acad. Sci. U.S.A., 100 (25): 14864-8. [PMID:14657352]

2. Jin, JL; O'Doherty, AM; Wang, S; Zheng, H; Sanders, KM; Yan, W. (2005) Catsper3 and catsper4 encode two cation channel-like proteins exclusively expressed in the testis. Biol. Reprod., 73 (6): 1235-42. [PMID:16107607]

3. Kirichok, Y; Navarro, B; Clapham, DE. (2006) Whole-cell patch-clamp measurements of spermatozoa reveal an alkaline-activated Ca2+ channel. Nature, 439 (7077): 737-40. [PMID:16467839]

4. Qi, H; Moran, MM; Navarro, B; Chong, JA; Krapivinsky, G; Krapivinsky, L; Kirichok, Y; Ramsey, IS; Quill, TA; Clapham, DE. (2007) All four CatSper ion channel proteins are required for male fertility and sperm cell hyperactivated motility. Proc. Natl. Acad. Sci. U.S.A., 104 (4): 1219-23. [PMID:17227845]

5. Quill, TA; Ren, D; Clapham, DE; Garbers, DL. (2001) A voltage-gated ion channel expressed specifically in spermatozoa. Proc. Natl. Acad. Sci. U.S.A., 98 (22): 12527-31. [PMID:11675491]

6. Ren, D; Navarro, B; Perez, G; Jackson, AC; Hsu, S; Shi, Q; Tilly, JL; Clapham, DE. (2001) A sperm ion channel required for sperm motility and male fertility. Nature, 413 (6856): 603-9. [PMID:11595941]

To cite this database page, please use the following:

David E. Clapham.
CatSper and Two-Pore channels: CatSper3. Last modified on 02/08/2010. Accessed on 20/05/2013. IUPHAR database (IUPHAR-DB), http://www.iuphar-db.org/DATABASE/ObjectDisplayForward?objectId=390.


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