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5-HT3B
| Previous and Unofficial Names |
| 5-HT3B |
| 5-HT3B |
| Structural Information |
| Species |
TM |
AA |
Chromosomal Location |
Gene Name |
Reference |
| Human |
4 |
441 |
11q23.1 |
HTR3B
|
22,32 |
| Rat |
4 |
437 |
8q23 |
Htr3b
|
89 |
| Mouse |
4 |
437 |
9 B |
Htr3b
|
89 |
|
|
| Database Links |
|
ChEMBL Target |
11305 (Hs), 11765 (Mm), 11885 (Rn) |
|
Ensembl |
ENSG00000149305 (Hs), ENSMUSG00000008590 (Mm), ENSRNOG00000006920 (Rn) |
|
Entrez Gene |
9177 (Hs), 57014 (Mm), 58963 (Rn) |
|
GeneCards |
HTR3B (Hs) |
|
HomoloGene |
38131 (Hs) |
|
OMIM |
604654 (Hs) |
|
PharmGKB Gene |
PA29556 (Hs) |
|
RefSeq Nucleotide |
NM_006028 (Hs), NM_020274 (Mm), NM_022189 (Rn) |
|
RefSeq Protein |
NP_006019 (Hs), NP_064670 (Mm), NP_071525 (Rn) |
|
UniGene Hs. |
241377 (Hs) |
|
UniProt |
O95264 (Hs), Q9JHJ5 (Mm), Q9JJ16 (Rn) |
|
Wikipedia |
5-HT3B |
| Search for 3D structures on the PDB |
|
Search using keywords: 5-HT3 receptors 5-HT3B
|
Search using accession numbers: Q9JJ16 || Q9JHJ5 || O95264
|
| Tissue Distribution
|
| Amygdala, caudate nucleus, hippocampus, thalamus. |
| Species: |
Human |
| Technique: |
Northern Blot |
| References: |
22 |
|
|
| Cerebral cortex including occipital, frontal and temporal regions, amygdala, hippocampus and testis. |
| Species: |
Human |
| Technique: |
PCR-based detection |
| References: |
32 |
|
|
| Amygdala, hippocampus, caudate nucleus, small intestine, colon and kidney. |
| Species: |
Human |
| Technique: |
RT-PCR |
| References: |
37 |
|
|
| Submucous plexus neurones. |
| Species: |
Human |
| Technique: |
Immunohistochemistry |
| References: |
81 |
|
|
| Brain, small intestine, colon. |
| Species: |
Mouse |
| Technique: |
RT-PCR |
| References: |
90 |
|
|
| Neurones of the myenteric and submucous plexus, mucosal epithelial cells of the intestinal crypts. |
| Species: |
Mouse |
| Technique: |
In situ hybridisation |
| References: |
81 |
|
|
| The 5-HT3B subunit is co-expressed with the 5-HT3A subunit in a subpopulation of superior cervical, nodose and dorsal root ganglion neurones. Nodose and dorsal root ganglion neurones co-expressing the 5-HT3A and 5-HT3B subunits have been demonstrated to project centrally to the nucleus tractus solitarius and superficial layers of the spinal cord, respectively. |
| Species: |
Rat |
| Technique: |
In situ hybridisation |
| References: |
96 |
|
|
| Restricted subpopulation of neurones in the pyramidal and molecular layers of the hippocampus. |
| Species: |
Rat |
| Technique: |
Immunohistochemistry |
| References: |
97 |
|
|
| Hippocampal interneurones. |
| Species: |
Rat |
| Technique: |
Immunohistochemistry |
| References: |
98 |
|
|
| Pyramidal neurones in the CA2, CA3 and to a lesser extent CA1 fields of the hippocampus. Large neurones in the hilus (CA4) of the hippocampus. |
| Species: |
Human |
| Technique: |
Immunohistochemistry |
| References: |
78 |
|
|
| Hippocampus (CA1 and dentate gyrus) and cerebral cortex. |
| Species: |
Rat |
| Technique: |
Immunohistochemistry |
| References: |
99 |
|
|
| Nodose, trigeminal, superior cervical and dorsal root ganglia. |
| Species: |
Rat |
| Technique: |
Immunohistochemistry |
| References: |
99 |
|
|
| Nodose, trigeminal, superior cervical and dorsal root ganglia. |
| Species: |
Mouse |
| Technique: |
Immunohistochemistry |
| References: |
99 |
|
|
| Physiological Consequences of Altering Gene Expression
|
| Despite the existence of 5-HT3B subunit mRNA in neuroblastoma cell lines, the introduction of the rat 5-HT3B subunit by transient transfection of mouse NB41A3 cells causes 5-HT to become less potent as an activator of 5-HT3 receptors native to the cell line and alters the kinetics of 5-HT activated currents. Introduction of the 5-HT3B subunit also abolishes the 5-HT induced increase in intracellular Ca2+ concentration seen in untransfected NB41A3 cells. |
| Species: |
Mouse |
| Tissue: |
NB41A3 cells |
| Technique: |
Transient transfection of the rat 5-HT3B subunit |
| References: |
60 |
|
|
| Clinically-Relevant Mutations
|
| Disease: |
Bipolar affective disease |
| OMIM: |
125480 |
| Comments: |
This deletion in the promotor region of the HTR3B gene is underrepresented in a sample of patients suffering from bipolar depression. |
| References: |
91 |
|
|
Click column headers to sort
|
| Deletion |
Human |
-100 to -102AAG |
91 |
|
|
| Disease: |
Major depression (female) |
| OMIM: |
608516 |
| Role: |
5-HT3B has been associated with major female depression in genetic analyses. |
| Comments: |
This polymorphism occurs at a significantly reduced frequency in female patients suffering from major depression and also patients with bipolar disorder, consistent with a protective influence of the variant allele. |
| References: |
92 |
|
|
Click column headers to sort
|
|
|
|
| Disease: |
Emesis. |
| Comments: |
This deletion variant in the promotor region of the HTR3B gene increases promoter activity in vitro and has been associated with an increased incidence of paroxetine-induced nausea, and with an increased frequency of vomiting caused by chemotherapy in patients receiving 5-HT3 receptor antagonists as anti-emetic therapy. |
| References: |
93-95 |
|
|
Click column headers to sort
|
| Deletion |
Human |
-100 to -102AAG |
94-95 |
|
|
| Gene Expression and Pathophysiology
|
| Downregulation of mRNA encoding the 5-HT3B subunit as a consequence of the knockout of SERT. |
| Tissue or cell type: |
Myenteric neurones |
| Pathophysiology: |
Downregulation of mRNA encoding the 5-HT3B subunit as a consequence of the knockout of SERT is associated with an increase in the EC50 for 5-HT in myenteric neurones. |
| Species: |
Mouse |
| Technique: |
Whole cell patch clamp. |
| References: |
90 |
|
|
| Biologically Significant Variants
|
| The human 5-HT3B(S156R) polymorphism causes enhanced functional responses to 5-HT mediated by heteromeric 5-HT3AB receptors. |
| Amino acids: |
441 |
| Type: |
Single nucleotide polymorphism |
| Species: |
Human |
| References: |
100 |
|
|
| The human 5-HT3B(Y129S) polymorphism causes decreased desensitisation, decreased inactivation rate, and a 7-fold increase in the mean channel open time of heteromeric 5-HT3AB receptors compared to receptors incorporating the Y129 variant. |
| Amino acids: |
441 |
| Type: |
Single nucleotide polymorphism |
| Species: |
Human |
| References: |
39 |
|
|
| The human 5-HT3B(I143T) polymorphism causes reduced functional responses to 5-HT mediated by heteromeric 5-HT3AB receptors and is associated with decreased cell surface expression. |
| Amino acids: |
441 |
| Type: |
Single nucleotide polymorphism |
| Species: |
Human |
| References: |
101 |
|
|
|
Subunit Comments |
|
The 5-HT3B subunit does not function as a homo-oligomer in recombinant expression systems and is retained in the endoplasmic reticulum [88]. Association with the 5-HT3A subunit facilitates trafficking of 5-HT3B to the plasma membrane as a heteromeric complex [88] with the suggested stoichiometry (5-HT3A)2(5-HT3B)3 [25]. Variants of the human 5-HT3B subunit canonical form have been postulated to exist in the intestine (the canonical form) and brain due to alternative promoters with the HTR3B gene that initiate transcription at different start sites [37]. One brain specific isoform (brain transcript 1) is predicted to differ from the canonical 5-HT3B subunit only within the signal peptide, whereas the second (brain transcript 2) is predicted to lack a large section of the extracellular N-terminal domain [37]. |
To cite this receptor data page, please use the following:
Sarah C. R. Lummis , Nicholas M. Barnes, Tim G. Hales, Beate Niesler, John A. Peters.
5-HT3 receptors: 5-HT3B. Last modified on 2010-07-01. Accessed on 2010-09-10. IUPHAR database (IUPHAR-DB), http://www.iuphar-db.org/DATABASE/ObjectDisplayForward?objectId=374.
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