Nomenclature: GPR116

Family: Adhesion Class GPCRs

Annotation status:  image of a green circle Annotated and expert reviewed. Please contact us if you can help with updates. 

Contents

Gene and Protein Information
Adhesion G protein-coupled receptor
Species TM AA Chromosomal Location Gene Symbol Gene Name Reference
Human 7 1346 6p12.3 GPR116 G protein-coupled receptor 116 6
Mouse 7 1348 17 B3 Gpr116 G protein-coupled receptor 116 8
Rat 7 1349 9q12 Gpr116 G protein-coupled receptor 116 1
Previous and Unofficial Names
FLJ90640
KPG_001
Ig-Hepta homolog
DKFZp564O1923
KIAA0758
Gprhep
Ig-Hepta
G protein-coupled hepta-helical receptor Ig-Hepta
G protein-coupled receptor 116
G-protein coupled hepta-helical receptor Ig-hepta
probable G-protein coupled receptor 116
8430401C09Rik
AI528491
Database Links
Ensembl Gene
Entrez Gene
GPCRDB
GeneCards
GenitoUrinary Development Molecular Anatomy Project
HomoloGene
Human Protein Reference Database
InterPro
KEGG Gene
PharmGKB Gene
PhosphoSitePlus
RefSeq Nucleotide
RefSeq Protein
TreeFam
UniGene Hs.
UniProtKB
Wikipedia
Agonist Comments
No ligands identified: orphan receptor.
Tissue Distribution
Unigene expression profile gives high normalized expression and multiple ESTs in thyroid, placenta, uterus, spleen, stomach, lung, kidney, brain, connective tissue, heart, eye, liver, muscle, pancreas, pharynx, testis.
Species:  Human
Technique: 
References: 
KIAA0758 highest in lung and kidney, then heart ovary, brain and skeletal muscle
Species:  Human
Technique:  RT-PCR.
References:  6
Endothelium, lung epithelium and lung adenocarcinoma cells
Species:  Human
Technique:  RT-PCR
References:  2,10
Embryonic kidney (glomeruli) and E18.5 brain microvessels
Species:  Mouse
Technique:  in situ hydridisation and RT-PCR
References:  4,10
Lung, liver, heart, kidney, testis, spleen, bonemarrow, skin, ovaries, thymus, adrenal gland
Species:  Mouse
Technique:  RT-PCR
References:  9
Mature adipocytes, stromal vascular cells
Species:  Mouse
Technique:  RT-PCR
References:  7
Very high expression in lung (alveolar walls); weak but detectable expression in kidney (cortex) and heart
Species:  Rat
Technique:  Northern blot and immunohistochemistry
References:  1
Expression Datasets

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Log average relative transcript abundance in mouse tissues measured by qPCR from Regard, J.B., Sato, I.T., and Coughlin, S.R. (2008). Anatomical profiling of G protein-coupled receptor expression. Cell, 135(3): 561-71. [PMID:18984166] [Raw data: website]

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Physiological Functions
Skin barrier assay
Species:  Mouse
Tissue:  Skin
References:  9
Physiological Consequences of Altering Gene Expression
Adipose-specific knockout mice showed a phenotype of exacerbated glucose intolerance and insulin resistance, hyperlipidemia, ectopic lipid accumulation, reduced adipocyte size, disregulated adipokine expression in adipocytes.
Species:  Mouse
Tissue:  Tissue/cell-type specific expression (Fabp4 promotor-driven)
Technique:  Gene knockouts
References:  7
General Comments
GPR116 is an orphan receptor that belongs to Family VI Adhesion-GPCRs together with GPR110, GPR111, GPR113 and GPR115 [3]. The genes of Family VI Adhesion-GPCRs except GPR113 are syntenically clustered on human chromosome 6 and mouse chromosome 17 suggesting the evolution from an ancestral gene through gene duplication and exon shuffling [9].

Full coding sequence human cDNA is publicly available, IMAGE:5298732 [5] in pBluescript vector. This clone has two known amino acid changing polymorphisms, rs586024 and rs547499. The Met604 and Thr856 alleles in this clone are both the more common allele in Europeans and Asians but the less common allele in sub-Saharan Africans.

REFERENCES

1. Abe J, Suzuki H, Notoya M, Yamamoto T, Hirose S. (1999) Ig-hepta, a novel member of the G protein-coupled hepta-helical receptor (GPCR) family that has immunoglobulin-like repeats in a long N-terminal extracellular domain and defines a new subfamily of GPCRs. J Biol Chem274: 19957-19964. [PMID:10391944]

2. Davidson B, Stavnes HT, Risberg B, Nesland JM, Wohlschlaeger J, Yang Y, Shih IeM, Wang TL. (2012) Gene expression signatures differentiate adenocarcinoma of lung and breast origin in effusions. Hum. Pathol.43 (5): 684-94. [PMID:21937081]

3. Fredriksson R, Gloriam DE, Höglund PJ, Lagerström MC, Schiöth HB. (2003) There exist at least 30 human G-protein-coupled receptors with long Ser/Thr-rich N-termini. Biochem. Biophys. Res. Commun.301 (3): 725-34. [PMID:12565841]

4. He L, Sun Y, Patrakka J, Mostad P, Norlin J, Xiao Z, Andrae J, Tryggvason K, Samuelsson T, Betsholtz C et al.. (2007) Glomerulus-specific mRNA transcripts and proteins identified through kidney expressed sequence tag database analysis. Kidney Int.71 (9): 889-900. [PMID:17332733]

5. Lennon G, Auffray C, Polymeropoulos M, Soares MB. (1996) The I.M.A.G.E. Consortium: an integrated molecular analysis of genomes and their expression. Genomics33: 151-152. [PMID:8617505]

6. Nagase T, Ishikawa K, Suyama M, Kikuno R, Miyajima N, Tanaka A, Kotani H, Nomura N, Ohara O. (1998) Prediction of the coding sequences of unidentified human genes. XI. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro. DNA Res5: 277-286. [PMID:9872452]

7. Nie T, Hui X, Gao X, Li K, Lin W, Xiang X, Ding M, Kuang Y, Xu A, Fei J et al.. (2012) Adipose tissue deletion of Gpr116 impairs insulin sensitivity through modulation of adipose function. FEBS Lett.586 (20): 3618-25. [PMID:22971422]

8. Okazaki N, F-Kikuno R, Ohara R, Inamoto S, Koseki H, Hiraoka S, Saga Y, Seino S, Nishimura M, Kaisho T, Hoshino K, Kitamura H, Nagase T, Ohara O, Koga H. (2004) Prediction of the coding sequences of mouse homologues of KIAA gene: IV. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries. DNA Res11: 205-218. [PMID:15368895]

9. Prömel S, Waller-Evans H, Dixon J, Zahn D, Colledge WH, Doran J, Carlton MB, Grosse J, Schöneberg T, Russ AP et al.. (2012) Characterization and functional study of a cluster of four highly conserved orphan adhesion-GPCR in mouse. Dev. Dyn.241 (10): 1591-602. [PMID:22837050]

10. Wallgard E, Larsson E, He L, Hellström M, Armulik A, Nisancioglu MH, Genove G, Lindahl P, Betsholtz C. (2008) Identification of a core set of 58 gene transcripts with broad and specific expression in the microvasculature. Arterioscler. Thromb. Vasc. Biol.28 (8): 1469-76. [PMID:18483404]

To cite this database page, please use the following:

Tobias Langenhan, Simone Prömel, Tom I. Bonner.
Adhesion Class GPCRs: GPR116. Last modified on 23/07/2013. Accessed on 01/11/2014. IUPHAR database (IUPHAR-DB), http://www.iuphar-db.org/DATABASE/ObjectDisplayForward?objectId=196.

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