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CELSR3

Family: Class B Orphans

Contents:
Gene and Protein Information
Previous and Unofficial Names
Database Links
Agonists
Transduction Mechanisms
Tissue Distribution
Expression Datasets
Physiological Functions
Physiological Consequences of Altering Gene Expression
Phenotypes, Alleles and Disease Models
Biologically Significant Variants
General Comments
References
Gene and Protein Information
class B G protein-coupled receptor
Species TM AA Chromosomal Location Gene Symbol Gene Name Reference
Human 7 3312 3p21.31B CELSR3 cadherin, EGF LAG seven-pass G-type receptor 3 (flamingo homolog, Drosophila) 16
Mouse 7 3301 9 F2 Celsr3 cadherin, EGF LAG seven-pass G-type receptor 3 (flamingo homolog, Drosophila) 13
Rat 7 3313 8q32 Celsr3 cadherin, EGF LAG seven-pass G-type receptor 3 (flamingo homolog, Drosophila) 7
Previous and Unofficial Names
cadherin, EGF LAG seven-pass G-type receptor 3 (flamingo homolog, Drosophila)
CDHF11
EGFL1
FMI1
HFMI1
MEGF2
RESDA1
Cadherin EGF LAG seven-pass G-type receptor 3
flamingo 1
hFmi1
multiple epidermal growth factor-like domains 2
epidermal growth factor-like 1
flamingo homolog 1
cadherin EGF LAG seven-pass G-type receptor 3, flamingo (Drosophila) homolog
multiple EGF-like domains protein 2
multiple epidermal growth factor-like domains protein 2
flamingo
Database Links
Ensembl ENSG00000008300 (Hs), ENSMUSG00000023473 (Mm), ENSRNOG00000034005 (Rn)
Entrez Gene 1951 (Hs), 107934 (Mm), 83466 (Rn)
GeneCards CELSR3 (Hs)
GenitoUrinary Development Molecular Anatomy Project Celsr3 (Mm)
HomoloGene 1077 (Hs)
Human Protein Reference Database 09179 (Hs)
InterPro Q9NYQ7 (Hs), Q91ZI0 (Mm), O88278 (Rn)
KEGG Gene hsa:1951 (Hs), mmu:107934 (Mm), rno:83466 (Rn)
OMIM 604264 (Hs)
PharmGKB Gene PA26395 (Hs)
PhosphoSitePlus Q9NYQ7 (Hs), Q91ZI0 (Mm), O88278 (Rn)
Protein Ontology (PRO) PRO:000005320 (Hs)
RefSeq Nucleotide NM_001407 (Hs), NM_080437 (Mm), NM_031320 (Rn)
RefSeq Protein NP_001398 (Hs), NP_536685 (Mm), NP_112610 (Rn)
TreeFam ENSG00000008300 (Hs), ENSMUSG00000023473 (Mm), ENSRNOG00000034005 (Rn)
UniGene Hs. 631926 (Hs)
UniProt Q9NYQ7 (Hs), Q91ZI0 (Mm), O88278 (Rn)
Wikipedia CELSR3
Orphan and other 7TM receptors
Agonist Comments
No ligands identified: orphan receptor.
Primary Transduction Mechanisms
Transducer Effector/Response
Other - See Comments
Comments:  Calcineurin [11] and Jun kinase [2].
References: 
Tissue Distribution
Central nervous system. Celsr3 is primarily expressed in differentiating neural cells after they exit the mitotic cycle, and drops to almost negligible levels when cell maturation is complete.
Species:  Mouse
Technique:  in situ hybridisation.
References:  6,8-10,13-15,17,19
Pancreas.
Species:  Mouse
Technique:  in situ hybridisation.
References:  2
Testes.
Species:  Rat
Technique:  in situ hybridisation.
References:  1
Expression Datasets

Click here to show/hide data

Log average relative transcript abundance in mouse tissues measured by qPCR from Regard, J.B., Sato, I.T., and Coughlin, S.R. (2008). Anatomical profiling of G protein-coupled receptor expression. Cell, 135(3): 561-71. [PMID:18984166] [Raw data: website]

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Physiological Functions
Inhibition of neurite outgrowth.
Species:  Rat
Tissue:  Hippocampus.
References:  11
Physiological Consequences of Altering Gene Expression
Mice with receptor knockout exhibit defects in tangential cortical interneuron migration.
Species:  Mouse
Tissue:  Brain.
Technique:  Gene knockout.
References:  17
Celsr3 inactivation resulted in marked malformation in the forebrain, absence of anterior commissure, and abnormalities in major axonal tracts. Celsr3 inactivation did not alter neuronal maturation. The neural phenotype was similar to that caused by inactivation of Frizzled3.
Species:  Mouse
Tissue: 
Technique:  Gene targeting in embryonic stem cells.
References:  12
Mice with receptor knockout exhibit defects in pancreatic β-cell differentiation.
Species:  Mouse
Tissue:  Pancreas.
Technique:  Gene knockout.
References:  2
Mice with receptor knockout exhibit defects in anterior-posterior axon tract organisation in the brainstem.
Species:  Mouse
Tissue:  Brainstem.
Technique:  Gene knockout.
References:  5
Mice with receptor knockout exhibit dysfunction of hippocampal connectivity and maturation and forebrain wiring.
Species:  Mouse
Tissue:  Hippocampus and forebrain.
Technique:  Gene knockout.
References:  4,18-19
Phenotypes, Alleles and Disease Models Mouse data from MGI

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Allele Composition & genetic background Accession Phenotype Id Phenotype Reference
Celsr3tm1Agof|Celsr3tm2Agof|Emx1+|Emx1tm1(cre)Krj Celsr3tm1Agof/Celsr3tm2Agof,Emx1tm1(cre)Krj/Emx1+
involves: 129P2/OlaHsd * 129S2/SvPas		 MGI:1858236  MGI:95387  MP:0008225 abnormal anterior commissure morphology PMID: 18487195 
Celsr3tm1Agof Celsr3tm1Agof/Celsr3tm1Agof
either: CD-1.129P2-Celsr3 or (involves: 129P2/OlaHsd * C57BL/6 * CD-1)		 MGI:1858236  MP:0002961 abnormal axon guidance PMID: 15778712 
Celsr3tm1Agof|Celsr3tm2Agof|Foxg1+|Foxg1tm1(cre)Skm Celsr3tm1Agof/Celsr3tm2Agof,Foxg1tm1(cre)Skm/Foxg1+
involves: 129P2/OlaHsd		 MGI:1347464  MGI:1858236  MP:0008128 abnormal brain internal capsule morphology PMID: 18487195 
Celsr3tm1Agof|Celsr3tm2Agof|Emx1+|Emx1tm1(cre)Krj Celsr3tm1Agof/Celsr3tm2Agof,Emx1tm1(cre)Krj/Emx1+
involves: 129P2/OlaHsd * 129S2/SvPas		 MGI:1858236  MGI:95387  MP:0008128 abnormal brain internal capsule morphology PMID: 18487195 
Celsr3tm1Agof|Celsr3tm2Agof|Tg(mI56i-cre,EGFP)1Kc Celsr3tm1Agof/Celsr3tm2Agof,Tg(mI56i-cre,EGFP)1Kc/0
involves: 129P2/OlaHsd		 MGI:1858236  MGI:3609984  MP:0008128 abnormal brain internal capsule morphology PMID: 18487195 
Celsr3tm1Agof Celsr3tm1Agof/Celsr3tm1Agof
either: CD-1.129P2-Celsr3 or (involves: 129P2/OlaHsd * C57BL/6 * CD-1)		 MGI:1858236  MP:0002152 abnormal brain morphology PMID: 15778712 
Celsr3tm1Agof Celsr3tm1Agof/Celsr3tm1Agof
either: CD-1.129P2-Celsr3 or (involves: 129P2/OlaHsd * C57BL/6 * CD-1)		 MGI:1858236  MP:0000788 abnormal cerebral cortex morphology PMID: 15778712 
Celsr3tm1Agof Celsr3tm1Agof/Celsr3tm1Agof
either: CD-1.129P2-Celsr3 or (involves: 129P2/OlaHsd * C57BL/6 * CD-1)		 MGI:1858236  MP:0000792 abnormal cortical marginal zone morphology PMID: 15778712 
Celsr3tm1Agof Celsr3tm1Agof/Celsr3tm1Agof
either: CD-1.129P2-Celsr3 or (involves: 129P2/OlaHsd * C57BL/6 * CD-1)		 MGI:1858236  MP:0002878 abnormal corticospinal tract morphology PMID: 15778712 
Celsr3tm1Agof|Celsr3tm2Agof|Foxg1+|Foxg1tm1(cre)Skm Celsr3tm1Agof/Celsr3tm2Agof,Foxg1tm1(cre)Skm/Foxg1+
involves: 129P2/OlaHsd		 MGI:1347464  MGI:1858236  MP:0002878 abnormal corticospinal tract morphology PMID: 18487195 
Celsr3tm1Agof|Celsr3tm2Agof|Emx1+|Emx1tm1(cre)Krj Celsr3tm1Agof/Celsr3tm2Agof,Emx1tm1(cre)Krj/Emx1+
involves: 129P2/OlaHsd * 129S2/SvPas		 MGI:1858236  MGI:95387  MP:0002878 abnormal corticospinal tract morphology PMID: 18487195 
Celsr3tm1Agof|Celsr3tm2Agof|Tg(mI56i-cre,EGFP)1Kc Celsr3tm1Agof/Celsr3tm2Agof,Tg(mI56i-cre,EGFP)1Kc/0
involves: 129P2/OlaHsd		 MGI:1858236  MGI:3609984  MP:0002878 abnormal corticospinal tract morphology PMID: 18487195 
Celsr3tm1Agof Celsr3tm1Agof/Celsr3tm1Agof
either: CD-1.129P2-Celsr3 or (involves: 129P2/OlaHsd * C57BL/6 * CD-1)		 MGI:1858236  MP:0003271 abnormal duodenum morphology PMID: 15778712 
Celsr3tm1Mrc Celsr3tm1Mrc/Celsr3tm1Mrc
either: (involves: 129S1/Sv * 129X1/SvJ) or (involves: 129S6/SvEvTac * C57BL/6NCr)		 MGI:1858236  MP:0004274 abnormal embryonic/fetal subventricular zone morphology PMID: 19332558 
Celsr3tm1Agof Celsr3tm1Agof/Celsr3tm1Agof
either: CD-1.129P2-Celsr3 or (involves: 129P2/OlaHsd * C57BL/6 * CD-1)		 MGI:1858236  MP:0004081 abnormal globus pallidus morphology PMID: 15778712 
Celsr3tm1Mrc Celsr3tm1Mrc/Celsr3tm1Mrc
either: (involves: 129S1/Sv * 129X1/SvJ) or (involves: 129S6/SvEvTac * C57BL/6NCr)		 MGI:1858236  MP:0004081 abnormal globus pallidus morphology PMID: 19332558 
Celsr3tm1Mrc Celsr3tm1Mrc/Celsr3tm1Mrc
either: (involves: 129S1/Sv * 129X1/SvJ) or (involves: 129S6/SvEvTac * C57BL/6NCr)		 MGI:1858236  MP:0002184 abnormal innervation PMID: 19332558 
Celsr3tm1Agof Celsr3tm1Agof/Celsr3tm1Agof
either: CD-1.129P2-Celsr3 or (involves: 129P2/OlaHsd * C57BL/6 * CD-1)		 MGI:1858236  MP:0001175 abnormal lung morphology PMID: 15778712 
Celsr3tm1Agof Celsr3tm1Agof/Celsr3tm1Agof
either: CD-1.129P2-Celsr3 or (involves: 129P2/OlaHsd * C57BL/6 * CD-1)		 MGI:1858236  MP:0000778 abnormal nervous system tract PMID: 15778712 
Celsr3tm1Mrc Celsr3tm1Mrc/Celsr3tm1Mrc
either: (involves: 129S1/Sv * 129X1/SvJ) or (involves: 129S6/SvEvTac * C57BL/6NCr)		 MGI:1858236  MP:0006009 abnormal neuronal migration PMID: 19332558 
Celsr3tm1Agof Celsr3tm1Agof/Celsr3tm1Agof
either: CD-1.129P2-Celsr3 or (involves: 129P2/OlaHsd * C57BL/6 * CD-1)		 MGI:1858236  MP:0002270 abnormal respiratory alveoli morphology PMID: 15778712 
Celsr3tm1Agof Celsr3tm1Agof/Celsr3tm1Agof
either: CD-1.129P2-Celsr3 or (involves: 129P2/OlaHsd * C57BL/6 * CD-1)		 MGI:1858236  MP:0000470 abnormal stomach morphology PMID: 15778712 
Celsr3tm1Agof Celsr3tm1Agof/Celsr3tm1Agof
either: CD-1.129P2-Celsr3 or (involves: 129P2/OlaHsd * C57BL/6 * CD-1)		 MGI:1858236  MP:0008227 absent anterior commissure PMID: 15778712 
Celsr3tm1Agof|Celsr3tm2Agof|Foxg1+|Foxg1tm1(cre)Skm Celsr3tm1Agof/Celsr3tm2Agof,Foxg1tm1(cre)Skm/Foxg1+
involves: 129P2/OlaHsd		 MGI:1347464  MGI:1858236  MP:0008227 absent anterior commissure PMID: 18487195 
Celsr3tm1Agof Celsr3tm1Agof/Celsr3tm1Agof
either: CD-1.129P2-Celsr3 or (involves: 129P2/OlaHsd * C57BL/6 * CD-1)		 MGI:1858236  MP:0008129 absent brain internal capsule PMID: 15778712 
Celsr3tm1Agof Celsr3tm1Agof/Celsr3tm1Agof
either: CD-1.129P2-Celsr3 or (involves: 129P2/OlaHsd * C57BL/6 * CD-1)		 MGI:1858236  MP:0001575 cyanosis PMID: 15778712 
Celsr3tm1Agof Celsr3tm1Agof/Celsr3tm1Agof
either: CD-1.129P2-Celsr3 or (involves: 129P2/OlaHsd * C57BL/6 * CD-1)		 MGI:1858236  MP:0001405 impaired coordination PMID: 15778712 
Celsr3tm1Agof Celsr3tm1Agof/Celsr3tm1Agof
either: CD-1.129P2-Celsr3 or (involves: 129P2/OlaHsd * C57BL/6 * CD-1)		 MGI:1858236  MP:0002058 neonatal lethality PMID: 15778712 
Celsr3tm1Mrc Celsr3tm1Mrc/Celsr3tm1Mrc
either: (involves: 129S1/Sv * 129X1/SvJ) or (involves: 129S6/SvEvTac * C57BL/6NCr)		 MGI:1858236  MP:0002058 neonatal lethality PMID: 19332558 
Celsr3tm1Agof Celsr3tm1Agof/Celsr3tm1Agof
either: CD-1.129P2-Celsr3 or (involves: 129P2/OlaHsd * C57BL/6 * CD-1)		 MGI:1858236  MP:0001953 respiratory failure PMID: 15778712 
Biologically Significant Variant Comments
Well characterised amino acid changing SNPs include rs12107252 (R1758Q), rs3821875 (T805S), and rs3733085 (P157A).
General Comments
A recent publication reveals an increase in Celsr3 expression in pancreatic tumour stellate cells [3]. Activation of these cells or their trans-differentiation is regulated by paracrine and autocrine loops of growth factors which are associated with pathological conditions such as liver injury, cirrhosis as well as cancer.

REFERENCES

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1. Beall, SA; Boekelheide, K; Johnson, KJ. (2005) Hybrid GPCR/cadherin (Celsr) proteins in rat testis are expressed with cell type specificity and exhibit differential Sertoli cell-germ cell adhesion activity. J. Androl., 26 (4): 529-38. [PMID:15955893]

2. Cortijo, C; Gouzi, M; Tissir, F; Grapin-Botton, A. (2012) Planar cell polarity controls pancreatic Beta cell differentiation and glucose homeostasis. Cell Rep, 2 (6): 1593-606. [PMID:23177622]

3. Erkan, M; Weis, N; Pan, Z; Schwager, C; Samkharadze, T; Jiang, X; Wirkner, U; Giese, NA; Ansorge, W; Debus, J; Huber, PE; Friess, H; Abdollahi, A; Kleeff, J. (2010) Organ-, inflammation- and cancer specific transcriptional fingerprints of pancreatic and hepatic stellate cells. Mol. Cancer, 9: 88. [PMID:20416094]

4. Feng, J; Xu, Y; Wang, M; Ruan, Y; So, KF; Tissir, F; Goffinet, A; Zhou, L. (2012) A role for atypical cadherin Celsr3 in hippocampal maturation and connectivity. J. Neurosci., 32 (40): 13729-43. [PMID:23035085]

5. Fenstermaker, AG; Prasad, AA; Bechara, A; Adolfs, Y; Tissir, F; Goffinet, A; Zou, Y; Pasterkamp, RJ. (2010) Wnt/planar cell polarity signaling controls the anterior-posterior organization of monoaminergic axons in the brainstem. J. Neurosci., 30 (47): 16053-64. [PMID:21106844]

6. Formstone, C. J. and Little, P. F. (2001) The flamingo-related mouse Celsr family (Celsr1-3) genes exhibit distinct patterns of expression during embryonic development. Mech Dev, 109: 91-94. [PMID:11677057]

7. Nakayama, M., Nakajima, D., Nagase, T., Nomura, N., Seki, N. and Ohara, O. (1998) Identification of high-molecular-weight proteins with multiple EGF-like motifs by motif-trap screening. Genomics, 51: 27-34. [PMID:9693030]

8. Qu, Y; Glasco, DM; Zhou, L; Sawant, A; Ravni, A; Fritzsch, B; Damrau, C; Murdoch, JN; Evans, S; Pfaff, SL; Formstone, C; Goffinet, AM; Chandrasekhar, A; Tissir, F. (2010) Atypical cadherins Celsr1-3 differentially regulate migration of facial branchiomotor neurons in mice. J. Neurosci., 30 (28): 9392-401. [PMID:20631168]

9. Shima, Y., Copeland, N. G., Gilbert, D. J., Jenkins, N. A., Chisaka, O., Takeichi, M. and Uemura, T. (2002) Differential expression of the seven-pass transmembrane cadherin genes Celsr1-3 and distribution of the Celsr2 protein during mouse development. Dev Dyn, 223: 321-332. [PMID:11891983]

10. Shima, Y., Kengaku, M., Hirano, T., Takeichi, M. and Uemura, T. (2004) Regulation of dendritic maintenance and growth by a mammalian 7-pass transmembrane cadherin. Dev Cell, 7: 205-216. [PMID:15296717]

11. Shima, Y; Kawaguchi, SY; Kosaka, K; Nakayama, M; Hoshino, M; Nabeshima, Y; Hirano, T; Uemura, T. (2007) Opposing roles in neurite growth control by two seven-pass transmembrane cadherins. Nat. Neurosci., 10 (8): 963-9. [PMID:17618280]

12. Tissir, F., Bar, I., Jossin, Y., De Backer, O. and Goffinet, A. M. (2005) Protocadherin Celsr3 is crucial in axonal tract development. Nat Neurosci, 8: 451-457. [PMID:15778712]

13. Tissir, F., De-Backer, O., Goffinet, A. M. and Lambert de Rouvroit, C. (2002) Developmental expression profiles of Celsr (Flamingo) genes in the mouse. Mech Dev, 112: 157-160. [PMID:11850187]

14. Tissir, F; Goffinet, AM. (2006) Expression of planar cell polarity genes during development of the mouse CNS. Eur. J. Neurosci., 23 (3): 597-607. [PMID:16487141]

15. Tissir, F; Qu, Y; Montcouquiol, M; Zhou, L; Komatsu, K; Shi, D; Fujimori, T; Labeau, J; Tyteca, D; Courtoy, P; Poumay, Y; Uemura, T; Goffinet, AM. (2010) Lack of cadherins Celsr2 and Celsr3 impairs ependymal ciliogenesis, leading to fatal hydrocephalus. Nat. Neurosci., 13 (6): 700-7. [PMID:20473291]

16. Wu, Q. and Maniatis, T. (2000) Large exons encoding multiple ectodomains are a characteristic feature of protocadherin genes. Proc Natl Acad Sci U S A, 97: 3124-3129. [PMID:10716726]

17. Ying, G; Wu, S; Hou, R; Huang, W; Capecchi, MR; Wu, Q. (2009) The protocadherin gene Celsr3 is required for interneuron migration in the mouse forebrain. Mol. Cell. Biol., 29 (11): 3045-61. [PMID:19332558]

18. Zhou, L; Bar, I; Achouri, Y; Campbell, K; De Backer, O; Hebert, JM; Jones, K; Kessaris, N; de Rouvroit, CL; O'Leary, D; Richardson, WD; Goffinet, AM; Tissir, F. (2008) Early forebrain wiring: genetic dissection using conditional Celsr3 mutant mice. Science, 320 (5878): 946-9. [PMID:18487195]

19. Zhou, L; Qu, Y; Tissir, F; Goffinet, AM. (2009) Role of the atypical cadherin Celsr3 during development of the internal capsule. Cereb. Cortex, 19 Suppl 1: i114-9. [PMID:19349379]

To cite this database page, please use the following:

Tom I. Bonner.
Class B Orphans: CELSR3. Last modified on 11/02/2013. Accessed on 19/05/2013. IUPHAR database (IUPHAR-DB), http://www.iuphar-db.org/DATABASE/ObjectDisplayForward?objectId=180.


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