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GPR182

Family: Class A Orphans

Contents:
Gene and Protein Information
Previous and Unofficial Names
Database Links
Agonists
Tissue Distribution
Expression Datasets
Physiological Functions
Physiological Consequences of Altering Gene Expression
Gene Expression and Pathophysiology
Biologically Significant Variants
References
Gene and Protein Information
class A G protein-coupled receptor
Species TM AA Chromosomal Location Gene Symbol Gene Name Reference
Human 7 404 12q13.3 GPR182 G protein-coupled receptor 182 2
Mouse 7 395 10 Gpr182 G protein-coupled receptor 182
Rat 7 395 7q22 Gpr182 G protein-coupled receptor 182
Previous and Unofficial Names
Names References
hrhAMR
AM-R
G10-D
Gpcr17
Gpcr22
MB10
NOW
ADMR
G-protein coupled receptor 182
GPR182
G10D
AMR
gamrh
adrenomedullin receptor
L1-R 12
G protein-coupled receptor 182
Database Links
ChEMBL Target 20021 (Hs)
Ensembl ENSG00000166856 (Hs), ENSMUSG00000058396 (Mm), ENSRNOG00000004311 (Rn)
Entrez Gene 11318 (Hs), 11536 (Mm), 29307 (Rn)
GeneCards GPR182 (Hs)
GenitoUrinary Development Molecular Anatomy Project Gpr182 (Mm)
HomoloGene 5255 (Hs)
Human Protein Reference Database 05605 (Hs)
InterPro O15218 (Hs), P43142 (Mm), P31392 (Rn)
KEGG Gene hsa:11318 (Hs), mmu:11536 (Mm), rno:29307 (Rn)
OMIM 605307 (Hs)
PharmGKB Gene PA162390165 (Hs)
PhosphoSitePlus O15218 (Hs), P43142 (Mm), P31392 (Rn)
Protein Ontology (PRO) PRO:000001419 (Hs)
RefSeq Nucleotide NM_007264 (Hs), NM_007412 (Mm), NM_053302 (Rn)
RefSeq Protein NP_009195 (Hs), NP_031438 (Mm), NP_445754 (Rn)
TreeFam ENSG00000166856 (Hs), ENSMUSG00000058396 (Mm), ENSRNOG00000004311 (Rn)
UniGene Hs. 483909 (Hs)
UniProt O15218 (Hs), P43142 (Mm), P31392 (Rn)
Wikipedia GPR182
Orphan and other 7TM receptors
Agonists
Key to terms and symbols Click column headers to sort
Ligand Sp. Action Affinity Units Reference
adrenomedullin {Sp: Rat} Rn Partial agonist 8.15 pEC50 5
Agonist Comments
Rat GPR182 was first proposed as adrenomedulin receptor [5]. However, it was later reported that rat and human GPR182 did not respond to adrenomedulin [6].
Tissue Distribution
Basal cells of the airway epithelium and Type II pneumocytes
Species:  Human
Technique:  in situ hybridisation
References:  8
Pancreatic cancer cells
Species:  Human
Technique:  RT-PCR and Western blotting
References:  11
High expression was detected in heart, skeletal muscle, liver, pancreas, stomach, spleen, lymph node, bone marrow, adrenal gland, tyroid. Low expression was detected in brain, lung, placenta, small intestine, thymus and leukocytes as well as fetal brain, lung, liver and kidney.
Species:  Human
Technique:  Northern blot
References:  2
Human pancreatic stellate cells (HPSCs) and human umbilical vein endothelial cells (HUVECs)
Species:  Human
Technique:  RT-PCR
References:  11-12
Skin epithelium in both normal and malignant conditions
Species:  Human
Technique:  in situ hybridisation, immunohistochemistry and RT-PCR
References:  7
Brain, heart, lung, adrenal gland and some tumor cell lines
Species:  Human
Technique:  RT-PCR
References:  9
Lung, adrenal, ovary, heart, spleen, cerebellum and cerebral cortex
Species:  Human
Technique:  RT-PCR
References:  11-12
Mouse lung endothelial cells (MLEC)
Species:  Mouse
Technique:  RT-PCR
References:  12
Mouse primitive placenta (especially in the giant trophoblastic cells), maturing chondrocytes
Species:  Mouse
Technique:  In situ hybridisation
References:  10
Mammary epithelial cells (luminal cells of ducts and alveoli, myoepithelial cells and cells comprising the terminal endbud), periductal fibroblasts
Species:  Mouse
Technique:  RT-PCR and in situ hybridisation
References:  3
Lung, liver, heart, spleen and kidney. Not detected in extraembryonic tissues.
Species:  Mouse
Technique:  Northern blot
References:  13
Lung, liver, adrenal, kidney, aorta, heart, spinal cord, gut, testes and brain.
Species:  Rat
Technique:  Northern blot
References:  1
Prostate
Species:  Rat
Technique:  RT-PCR
References:  4
Rat embryonic tissue (whole embryo, placenta, yolk sac, heart, lung, liver, brain and gut)
Species:  Rat
Technique:  RT-PCR
References:  10
Tissue Distribution Comments
It is reported that the expression level of GPR182 in mouse embrynonic tissue was low at 7 and 11 d.p.c, but drastically increased by 15 d.p.c. and stayed high until 17 d.p.c. [13]. In contrast to the previous report [10], Yotsumoto et al. did not detect the expression of GPR182 in the giant trophoblatic cells by Northern blot analyses [13]. It is found that the expression pattern of GPR182 in human tissue is considerably different from that of rat tissues [2].
Expression Datasets

Click here to show/hide data

Log average relative transcript abundance in mouse tissues measured by qPCR from Regard, J.B., Sato, I.T., and Coughlin, S.R. (2008). Anatomical profiling of G protein-coupled receptor expression. Cell, 135(3): 561-71. [PMID:18984166] [Raw data: website]

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Physiological Functions
Mediate autocrine action of adrenomedulin in cell proliferation.
Species:  Human
Tissue:  Pancreatic cancer cell lines
References:  11
Physiological Consequences of Altering Gene Expression
ShRNA-mediated silencing of GPR182 reduces tumour growth and metastasis in vivo.
Species:  Human
Tissue:  Pancreatic cancer cells
Technique:  RNA interference
References:  12
SiRNA-mediated silencing of GPR182 reduces the cell growth.
Species:  Human
Tissue:  Human pancreatic stellate cells (HPSC) and human umbilical vein endothelial cells (HUVEC)
Technique:  RNA interference
References:  12
ShRNA-mediated silencing of GPR182 strongly reduces tumour growth and metastasis in vivo.
Species:  Mouse
Tissue:  Lung and liver
Technique:  RNA interference
References:  12
Silencing of GPR182 inhibits basal- and adrenomedullin-induced growth and invasion in pancreatic cancer cells.
Species:  Human
Tissue:  Pancreatic cancer cells
Technique:  RNA interference
References:  11
SiRNA-mediated silencing of GPR182 reduces the cell growth.
Species:  Mouse
Tissue:  Mouse lung endothelial cells (MLEC)
Technique:  RNA interference
References:  12
SiRNA-mediated silencing of GPR182 abolishes adrenomedullin-mediated tube formation.
Species:  Human
Tissue:  Human umbilical vein endothelial cells (HUVEC)
Technique:  RNA interference
References:  12
SiRNA-mediated silencing of GPR182 abolishes adrenomedullin-mediated tube formation.
Species:  Mouse
Tissue:  Mouse lung endothelial cells (MLEC)
Technique:  RNA interference
References:  12
Gene Expression and Pathophysiology Comments
It is proposed that adrenomedullin/GPR182 may involve in respiratory physiology and lung carcinogenesis [8].
Biologically Significant Variants
C349R
SNP accession:  rs35493121 
Type:  Naturally occurring SNPs.
Species:  Human
References: 
Available Assays
DiscoveRx PathHunter® CHO-K1 ADMR (GPR182) (Orphan) High Expression β-Arrestin Cell Line Human Cat No. 93-0403C2

REFERENCES

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1. Harrison, JK; Barber, CM; Lynch, KR. (1993) Molecular cloning of a novel rat G-protein-coupled receptor gene expressed prominently in lung, adrenal, and liver. FEBS Lett., 318 (1): 17-22. [PMID:8382168]

2. Hänze, J; Dittrich, K; Dötsch, J; Rascher, W. (1997) Molecular cloning of a novel human receptor gene with homology to the rat adrenomedullin receptor and high expression in heart and immune system. Biochem. Biophys. Res. Commun., 240 (1): 183-8. [PMID:9367907]

3. Jahnke, GD; Miller, MJ; Martínez, A; Montuenga, L; Cuttitta, F. (1997) Adrenomedullin expression in the mouse mammary gland: evidence for the mature form in milk. J. Mol. Endocrinol., 19 (3): 279-89. [PMID:9460649]

4. Jiménez, N; Calvo, A; Martínez, A; Rosell, D; Cuttitta, F; Montuenga, LM. (1999) Expression of adrenomedullin and proadrenomedullin N-terminal 20 peptide in human and rat prostate. J. Histochem. Cytochem., 47 (9): 1167-78. [PMID:10449538]

5. Kapas, S; Catt, KJ; Clark, AJ. (1995) Cloning and expression of cDNA encoding a rat adrenomedullin receptor. J. Biol. Chem., 270 (43): 25344-7. [PMID:7592696]

6. Kennedy, S. P., Sun, D., Oleynek, J. J., Hoth, C. F., Kong, J. and Hill, R. J. (1998) Expression of the rat adrenomedullin receptor or a putative human adrenomedullin receptor does not correlate with adrenomedullin binding or functional response. Biochem Biophys Res Commun, 244: 832-837. [PMID:9535752]

7. Martínez, A; Elsasser, TH; Muro-Cacho, C; Moody, TW; Miller, MJ; Macri, CJ; Cuttitta, F. (1997) Expression of adrenomedullin and its receptor in normal and malignant human skin: a potential pluripotent role in the integument. Endocrinology, 138 (12): 5597-604. [PMID:9389548]

8. Martínez, A; Miller, MJ; Catt, KJ; Cuttitta, F. (1997) Adrenomedullin receptor expression in human lung and in pulmonary tumors. J. Histochem. Cytochem., 45 (2): 159-64. [PMID:9016306]

9. Miller, MJ; Martínez, A; Unsworth, EJ; Thiele, CJ; Moody, TW; Elsasser, T; Cuttitta, F. (1996) Adrenomedullin expression in human tumor cell lines. Its potential role as an autocrine growth factor. J. Biol. Chem., 271 (38): 23345-51. [PMID:8798536]

10. Montuenga, LM; Martínez, A; Miller, MJ; Unsworth, EJ; Cuttitta, F. (1997) Expression of adrenomedullin and its receptor during embryogenesis suggests autocrine or paracrine modes of action. Endocrinology, 138 (1): 440-51. [PMID:8977434]

11. Ramachandran, V; Arumugam, T; Hwang, RF; Greenson, JK; Simeone, DM; Logsdon, CD. (2007) Adrenomedullin is expressed in pancreatic cancer and stimulates cell proliferation and invasion in an autocrine manner via the adrenomedullin receptor, ADMR. Cancer Res., 67 (6): 2666-75. [PMID:17363587]

12. Ramachandran, V; Arumugam, T; Langley, R; Hwang, RF; Vivas-Mejia, P; Sood, AK; Lopez-Berestein, G; Logsdon, CD. (2009) The ADMR receptor mediates the effects of adrenomedullin on pancreatic cancer cells and on cells of the tumor microenvironment. PLoS ONE, 4 (10): e7502. [PMID:19847298]

13. Yotsumoto, S; Shimada, T; Cui, CY; Nakashima, H; Fujiwara, H; Ko, MS. (1998) Expression of adrenomedullin, a hypotensive peptide, in the trophoblast giant cells at the embryo implantation site in mouse. Dev. Biol., 203 (2): 264-75. [PMID:9808778]

To cite this database page, please use the following:

Wen Chiy Liew.
Class A Orphans: GPR182. Last modified on 05/11/2012. Accessed on 22/05/2013. IUPHAR database (IUPHAR-DB), http://www.iuphar-db.org/DATABASE/ObjectDisplayForward?objectId=146.


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