Nomenclature: GPR85

Family: Class A Orphans

Annotation status:  image of a green circle Annotated and expert reviewed. Please contact us if you can help with updates. 

Contents

Gene and Protein Information
class A G protein-coupled receptor
Species TM AA Chromosomal Location Gene Symbol Gene Name Reference
Human 7 370 7q31 GPR85 G protein-coupled receptor 85 2
Mouse 7 370 6 A1 Gpr85 G protein-coupled receptor 85 2
Rat 7 370 4q21 Gpr85 G protein-coupled receptor 85 2
Previous and Unofficial Names
Super Conserved Receptor Expressed in Brain 2
Srep2
MGC105281
SREB2
GPR85
G protein-coupled receptor 85
Super conserved receptor expressed in brain 2
seven transmembrane helix receptor
probable G-protein coupled receptor 85
SREB
PKrCx1
2900026B03Rik
rgpr85
Database Links
Ensembl Gene
Entrez Gene
GPCRDB
GeneCards
GenitoUrinary Development Molecular Anatomy Project
HomoloGene
Human Protein Reference Database
InterPro
KEGG Gene
OMIM
PharmGKB Gene
PhosphoSitePlus
Protein Ontology (PRO)
RefSeq Nucleotide
RefSeq Protein
TreeFam
UniGene Hs.
UniProtKB
Wikipedia
Tissue Distribution
Brain, testis, small intestine, placenta, spleen
Species:  Human
Technique:  Northern blot
References:  2,8
Forebrain
Species:  Human
Technique:  In situ hybridization
References:  7
Neural tissue and macrophages
Species:  Mouse
Technique:  Microarray analysis
References:  6
Upper intestine (muscle-mesenteric nerve layer)
Species:  Mouse
Technique:  RT-PCR
References:  4
Forebrain
Species:  Mouse
Technique:  Northern blot
References:  2-3
Brain and spinal cord
Species:  Rat
Technique:  In situ hybridization
References:  5,7
Expression Datasets

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Log average relative transcript abundance in mouse tissues measured by qPCR from Regard, J.B., Sato, I.T., and Coughlin, S.R. (2008). Anatomical profiling of G protein-coupled receptor expression. Cell, 135(3): 561-71. [PMID:18984166] [Raw data: website]

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Physiological Functions
Negative regulation of hippocampal adult neurogenesis and neurogenesis dependent learning and memory
Species:  Mouse
Tissue:  Hippocampus(dentate gyrus)
References:  1
Physiological Consequences of Altering Gene Expression
Mice with receptor knockout show enhanced new neuronal survival in dentate gyrus, enhanced ability to discriminate spatial relationships, and significant increase in brain weight.
Species:  Mouse
Tissue:  Brain
Technique:  Gene knockout
References:  1,9
Receptor overexpression leads to decreased new cell proliferation and survival in the dentate gyrus. Morphology deficits in newly generated neurons are seem with ventricular enlargement and behaviour mirroring psychiatric disorders. Mice have decreased ability to discriminate spatial relationships and a reciprocal phenotype to knock out mice in Y-maze working memory tasks. Decreased social interaction, abnormal sensorimotor gating and impaired memory are also seen.
Species:  Mouse
Tissue:  Brain
Technique:  Gene over-expression
References:  1,9
Phenotypes, Alleles and Disease Models Mouse data from MGI

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Allele Composition & genetic background Accession Phenotype Id Phenotype Reference
Gpr85tm1Mima Gpr85tm1Mima/Gpr85tm1Mima
involves: C57BL/6 * CBA
MGI:1927851  MP:0001469 abnormal contextual conditioning behavior PMID: 18413613 
Gpr85tm1Mima Gpr85tm1Mima/Gpr85tm1Mima
involves: C57BL/6 * CBA
MGI:1927851  MP:0001260 increased body weight PMID: 18413613 
Gpr85tm1Mima Gpr85tm1Mima/Gpr85tm1Mima
involves: C57BL/6 * CBA
MGI:1927851  MP:0005238 increased brain size PMID: 18413613 
Gpr85tm1Mima Gpr85tm1Mima/Gpr85tm1Mima
involves: C57BL/6 * CBA
MGI:1927851  MP:0002176 increased brain weight PMID: 18413613 
Clinically-Relevant Mutations and Pathophysiology Comments
3 missense SNPs have been linked to schizophrenia, though are not confirmed as causative: S104F (rs201803332), Y57C (rs201795714) and V46L (rs148433444) [1].
Gene Expression and Pathophysiology Comments
Mouse models with receptor overexpression have been linked to schizophrenia [1].
Available Assays
DiscoveRx PathHunter® CHO-K1 GPR85 β-Arrestin Orphan GPCR Cell Line (Cat no. 93-0409C2A)
PathHunter® eXpress GPR85 CHO-K1 β-Arrestin Orphan GPCR Assay (Cat no. 93-0409E2ACP1M)
PathHunter® eXpress GPR85 U2OS β-Arrestin Orphan GPCR Assay (Cat no. 93-0409E3CP1M)
PathHunter® U2OS GPR85 β-Arrestin Orphan GPCR Cell Line (Cat no. 93-0409C3)
more info

REFERENCES

1. Chen Q, Kogan JH, Gross AK, Zhou Y, Walton NM, Shin R, Heusner CL, Miyake S, Tajinda K, Tamura K et al.. (2012) SREB2/GPR85, a schizophrenia risk factor, negatively regulates hippocampal adult neurogenesis and neurogenesis-dependent learning and memory. Eur J Neurosci,  [Epub ahead of print]. [PMID:22697179]

2. Hellebrand S, Schaller HC, Wittenberger T. (2000) The brain-specific G-protein coupled receptor GPR85 with identical protein sequence in man and mouse maps to human chromosome 7q31. Biochim. Biophys. Acta1493 (1-2): 269-72. [PMID:10978537]

3. Hellebrand S, Wittenberger T, Schaller HC, Hermans-Borgmeyer I. (2001) Gpr85, a novel member of the G-protein coupled receptor family, prominently expressed in the developing mouse cerebral cortex. Brain Res. Gene Expr. Patterns1 (1): 13-6. [PMID:15018813]

4. Ito J, Ito M, Nambu H, Fujikawa T, Tanaka K, Iwaasa H, Tokita S. (2009) Anatomical and histological profiling of orphan G-protein-coupled receptor expression in gastrointestinal tract of C57BL/6J mice. Cell Tissue Res.338 (2): 257-69. [PMID:19763624]

5. Jeon J, Kim C, Sun W, Chung H, Park SH, Kim H. (2002) Cloning and localization of rgpr85 encoding rat G-protein-coupled receptor. Biochem. Biophys. Res. Commun.298 (4): 613-8. [PMID:12408996]

6. Lattin JE, Schroder K, Su AI, Walker JR, Zhang J, Wiltshire T, Saijo K, Glass CK, Hume DA, Kellie S, Sweet MJ. (2008) Expression analysis of G Protein-Coupled Receptors in mouse macrophages. Immunome Res4: 5. [PMID:18442421]

7. Matsumoto M, Beltaifa S, Weickert CS, Herman MM, Hyde TM, Saunders RC, Lipska BK, Weinberger DR, Kleinman JE. (2005) A conserved mRNA expression profile of SREB2 (GPR85) in adult human, monkey, and rat forebrain. Brain Res. Mol. Brain Res.138 (1): 58-69. [PMID:15893849]

8. Matsumoto M, Saito T, Takasaki J, Kamohara M, Sugimoto T, Kobayashi M, Tadokoro M, Matsumoto S, Ohishi T, Furuichi K. (2000) An evolutionarily conserved G-protein coupled receptor family, SREB, expressed in the central nervous system. Biochem. Biophys. Res. Commun.272 (2): 576-82. [PMID:10833454]

9. Matsumoto M, Straub RE, Marenco S, Nicodemus KK, Matsumoto S, Fujikawa A, Miyoshi S, Shobo M, Takahashi S, Yarimizu J, Yuri M, Hiramoto M, Morita S, Yokota H, Sasayama T, Terai K, Yoshino M, Miyake A, Callicott JH, Egan MF, Meyer-Lindenberg A, Kempf L, Honea R, Vakkalanka RK, Takasaki J, Kamohara M, Soga T, Hiyama H, Ishii H, Matsuo A, Nishimura S, Matsuoka N, Kobori M, Matsushime H, Katoh M, Furuichi K, Weinberger DR. (2008) The evolutionarily conserved G protein-coupled receptor SREB2/GPR85 influences brain size, behavior, and vulnerability to schizophrenia. Proc. Natl. Acad. Sci. U.S.A.105 (16): 6133-8. [PMID:18413613]

To cite this database page, please use the following:

Anthony P. Davenport, Stephen Alexander, Joanna L. Sharman, Adam J. Pawson, Helen E. Benson, Amy E. Monaghan, Wen Chiy Liew, Chido Mpamhanga, Jim Battey, Richard V. Benya, Robert T. Jensen, Sadashiva Karnik, Evi Kostenis, Eliot Spindel, Laura Storjohann, Kalyan Tirupula, Tom I. Bonner, Richard Neubig, Jean-Philippe Pin, Michael Spedding, Anthony Harmar.
Class A Orphans: GPR85. Last modified on 26/11/2013. Accessed on 22/10/2014. IUPHAR database (IUPHAR-DB), http://www.iuphar-db.org/DATABASE/ObjectDisplayForward?objectId=121.

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