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TRPM5

Family: Transient Receptor Potential channels

Contents:
Gene and Protein Information
Previous and Unofficial Names
Database Links
Ion Selectivity and Conductance
Voltage Dependence
Activators
Pore Blockers
Tissue Distribution
Functional Assays
Physiological Functions
Phenotypes, Alleles and Disease Models
References
Gene and Protein Information
Species TM P Loops AA Chromosomal Location Gene Symbol Gene Name Reference
Human 6 1 1165 11p15.5 TRPM5 transient receptor potential cation channel, subfamily M, member 5 9
Mouse 6 1 1158 7 F5 Trpm5 transient receptor potential cation channel, subfamily M, member 5 2
Rat 6 1 1158 1q42 Trpm5 transient receptor potential cation channel, subfamily M, member 5
Previous and Unofficial Names
MTR1
TRP-T
LTRPC5
LOC365391
Trpm5_predicted
transient receptor potential cation channel subfamily M member 5
transient receptor potential cation channel, subfamily M, member 5
transient receptor potential cation channel, subfamily M, member 5 (predicted)
9430099A16Rik
Database Links
Ensembl ENSG00000070985 (Hs), ENSMUSG00000009246 (Mm), ENSRNOG00000020484 (Rn)
Entrez Gene 29850 (Hs), 56843 (Mm), 365391 (Rn)
GeneCards TRPM5 (Hs)
GenitoUrinary Development Molecular Anatomy Project Trpm5 (Mm)
HomoloGene 22818 (Hs), 22818
Human Protein Reference Database 16064 (Hs)
InterPro Q9NZQ8 (Hs), Q9JJH7 (Mm)
KEGG Gene hsa:29850 (Hs), mmu:56843 (Mm), rno:365391 (Rn)
OMIM 604600 (Hs)
PharmGKB Gene PA37869 (Hs)
PhosphoSitePlus Q9NZQ8 (Hs), Q9JJH7 (Mm)
Protein Ontology (PRO) PRO:000016701 (Hs)
RefSeq Nucleotide NM_014555 (Hs), NM_020277 (Mm), NM_001191896 (Rn)
RefSeq Protein NP_055370 (Hs), NP_064673 (Mm), NP_001178825 (Rn)
TreeFam ENSG00000070985 (Hs), ENSMUSG00000009246 (Mm), ENSRNOG00000020484 (Rn)
UniGene Hs. 272287 (Hs)
UniProt Q9NZQ8 (Hs), Q9JJH7 (Mm)
Wikipedia TRPM5
Transient Receptor Potential channels
Search for 3D structures on the PDB
Search by keyword: Transient Receptor Potential channels TRPM5 Search by accession number: Q9JJH7, Q9NZQ8
Ion Selectivity and Conductance Comments
Nonselective for monovalent cations, impermeable to divalent cations. Average single-channel conductance of mTRPM5 is 23 pS [3].
Voltage Dependence
  V0.5 (mV)  τ (msec)  Reference  Cell type  Species 
Activation  - 1.9 12 HEK 293 cells. Mouse
Inactivation  - -
Comments  Heat activation of TRPM5 is the result of a drastic leftward shift of the voltage dependence of activation (-7.0 mV.°C-1) [11].
Physical activators (Human)
membrane depolarization (V½ = 0 to + 120 mV dependent upon conditions), heat (Q10 = 10.3 @ -75 mV between 15 and 25°C)
Activators
Key to terms and symbols Click column headers to sort
Ligand Sp. Affinity Units Concentration range (M) Holding voltage (mV) Reference
Cai2+ Mm 4.2 – 6.2 (median: 4.7) pEC50 - -80.0 – 80.0 3-4,12
Activator Comments
Desensitisation of TRPM5 is modulated by PIP2, which acts as a co-factor for activation [4].
Pore Blocker Comments
TRPM5 is sensitive to pH below 7.0, with complete block at around pH 5.9 [5].
Tissue Distribution
Taste tissue, stomach, intestines, uterus, testes.
Species:  Mouse
Technique:  Immunocytochemistry, Northern blot, in situ hybridisation.
References:  8,13
Functional Assays
Patch clamp (whole-cell and single-channel recordings).
Species:  Mouse
Tissue:  HEK 293 or CHO cells transfected with TRPM5 vector.
Response measured:  Ca2+ evoked currents.
References:  3-4,6-8,10-13
Two-electrode voltage clamp technique.
Species:  Mouse
Tissue:  Xenopus laevis oocytes injected with TRPM5 cDNA.
Response measured:  Ca2+ evoked currents.
References:  8
Physiological Functions
Taste receptor channel.
Species:  Mouse
Tissue:  Taste tissue.
References:  1,8,11,13
Phenotypes, Alleles and Disease Models Mouse data from MGI

Click here to show/hide data

Allele Composition & genetic background Accession Phenotype Id Phenotype Reference
Trpm5tm1Csz Trpm5tm1Csz/Trpm5tm1Csz
involves: 129S1/Sv * 129X1/SvJ * C57BL/6		 MGI:1861718  MP:0004210 abnormal bitter taste sensitivity PMID: 12581520 
Trpm5tm1Sdmk Trpm5tm1Sdmk/Trpm5tm1Sdmk
involves: C57BL/6		 MGI:1861718  MP:0004210 abnormal bitter taste sensitivity PMID: 16355226 
Trpm4tm1.1Knt|Trpm5tm1Csz Trpm4tm1.1Knt/Trpm4tm1.1Knt,Trpm5tm1Csz/Trpm5tm1Csz
involves: 129S1/Sv * 129X1/SvJ		 MGI:1861718  MGI:1915917  MP:0002376 abnormal dendritic cell physiology PMID: 18758465 
Trpm5tm1Csz Trpm5tm1Csz/Trpm5tm1Csz
involves: 129S1/Sv * 129X1/SvJ * C57BL/6		 MGI:1861718  MP:0001985 abnormal gustatory system physiology PMID: 12581520 
Trpm5tm1Sdmk Trpm5tm1Sdmk/Trpm5tm1Sdmk
involves: C57BL/6		 MGI:1861718  MP:0004212 abnormal salty taste sensitivity PMID: 16355226 
Trpm5tm1Sdmk Trpm5tm1Sdmk/Trpm5tm1Sdmk
involves: C57BL/6		 MGI:1861718  MP:0004211 abnormal sour taste sensitivity PMID: 16355226 
Trpm5tm1Csz Trpm5tm1Csz/Trpm5tm1Csz
involves: 129S1/Sv * 129X1/SvJ * C57BL/6		 MGI:1861718  MP:0004209 abnormal sweet taste sensitivity PMID: 12581520 
Trpm5tm1Sdmk Trpm5tm1Sdmk/Trpm5tm1Sdmk
involves: C57BL/6		 MGI:1861718  MP:0004209 abnormal sweet taste sensitivity PMID: 16355226 
Trpm5tm1Csz Trpm5tm1Csz/Trpm5tm1Csz
involves: 129S1/Sv * 129X1/SvJ * C57BL/6		 MGI:1861718  MP:0001986 abnormal taste sensitivity PMID: 12581520 
Trpm5tm1Sdmk Trpm5tm1Sdmk/Trpm5tm1Sdmk
involves: C57BL/6		 MGI:1861718  MP:0001986 abnormal taste sensitivity PMID: 16355226 
Trpm5tm1Dgen Trpm5tm1Dgen/Trpm5tm1Dgen
B6.129P2-Trpm5		 MGI:1861718  MP:0001986 abnormal taste sensitivity PMID: 19244541 
Trpm5tm1Sdmk Trpm5tm1Sdmk/Trpm5tm1Sdmk
involves: C57BL/6		 MGI:1861718  MP:0004213 abnormal unami taste sensitivity PMID: 15738181  16355226 
Trpm4tm1.1Knt|Trpm5tm1Csz Trpm4tm1.1Knt/Trpm4tm1.1Knt,Trpm5tm1Csz/Trpm5tm1Csz
involves: 129S1/Sv * 129X1/SvJ		 MGI:1861718  MGI:1915917  MP:0008127 decreased dendritic cell number PMID: 18758465 
Trpm5tm1Dgen Trpm5tm1Dgen/Trpm5tm1Dgen
involves: 129P2/OlaHsd * C57BL/6		 MGI:1861718  MP:0002169 no abnormal phenotype detected

REFERENCES

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1. Damak, S; Rong, M; Yasumatsu, K; Kokrashvili, Z; Pérez, CA; Shigemura, N; Yoshida, R; Mosinger, B; Glendinning, JI; Ninomiya, Y; Margolskee, RF. (2006) Trpm5 null mice respond to bitter, sweet, and umami compounds. Chem. Senses, 31 (3): 253-64. [PMID:16436689]

2. Enklaar, T; Esswein, M; Oswald, M; Hilbert, K; Winterpacht, A; Higgins, M; Zabel, B; Prawitt, D. (2000) Mtr1, a novel biallelically expressed gene in the center of the mouse distal chromosome 7 imprinting cluster, is a member of the Trp gene family. Genomics, 67 (2): 179-87. [PMID:10903843]

3. Hofmann, T; Chubanov, V; Gudermann, T; Montell, C. (2003) TRPM5 is a voltage-modulated and Ca(2+)-activated monovalent selective cation channel. Curr. Biol., 13 (13): 1153-8. [PMID:12842017]

4. Liu, D; Liman, ER. (2003) Intracellular Ca2+ and the phospholipid PIP2 regulate the taste transduction ion channel TRPM5. Proc. Natl. Acad. Sci. U.S.A., 100 (25): 15160-5. [PMID:14657398]

5. Liu, D; Zhang, Z; Liman, ER. (2005) Extracellular acid block and acid-enhanced inactivation of the Ca2+-activated cation channel TRPM5 involve residues in the S3-S4 and S5-S6 extracellular domains. J. Biol. Chem., 280 (21): 20691-9. [PMID:15731110]

6. Nilius, B; Prenen, J; Janssens, A; Owsianik, G; Wang, C; Zhu, MX; Voets, T. (2005) The selectivity filter of the cation channel TRPM4. J. Biol. Chem., 280 (24): 22899-906. [PMID:15845551]

7. Nilius, B; Prenen, J; Janssens, A; Voets, T; Droogmans, G. (2004) Decavanadate modulates gating of TRPM4 cation channels. J. Physiol. (Lond.), 560 (Pt 3): 753-65. [PMID:15331675]

8. Perez, CA; Huang, L; Rong, M; Kozak, JA; Preuss, AK; Zhang, H; Max, M; Margolskee, RF. (2002) A transient receptor potential channel expressed in taste receptor cells. Nat. Neurosci., 5 (11): 1169-76. [PMID:12368808]

9. Prawitt, D; Enklaar, T; Klemm, G; Gärtner, B; Spangenberg, C; Winterpacht, A; Higgins, M; Pelletier, J; Zabel, B. (2000) Identification and characterization of MTR1, a novel gene with homology to melastatin (MLSN1) and the trp gene family located in the BWS-WT2 critical region on chromosome 11p15.5 and showing allele-specific expression. Hum. Mol. Genet., 9 (2): 203-16. [PMID:10607831]

10. Prawitt, D; Monteilh-Zoller, MK; Brixel, L; Spangenberg, C; Zabel, B; Fleig, A; Penner, R. (2003) TRPM5 is a transient Ca2+-activated cation channel responding to rapid changes in [Ca2+]i. Proc. Natl. Acad. Sci. U.S.A., 100 (25): 15166-71. [PMID:14634208]

11. Talavera, K; Yasumatsu, K; Voets, T; Droogmans, G; Shigemura, N; Ninomiya, Y; Margolskee, RF; Nilius, B. (2005) Heat activation of TRPM5 underlies thermal sensitivity of sweet taste. Nature, 438 (7070): 1022-5. [PMID:16355226]

12. Ullrich, ND; Voets, T; Prenen, J; Vennekens, R; Talavera, K; Droogmans, G; Nilius, B. (2005) Comparison of functional properties of the Ca2+-activated cation channels TRPM4 and TRPM5 from mice. Cell Calcium, 37 (3): 267-78. [PMID:15670874]

13. Zhang, Y; Hoon, MA; Chandrashekar, J; Mueller, KL; Cook, B; Wu, D; Zuker, CS; Ryba, NJ. (2003) Coding of sweet, bitter, and umami tastes: different receptor cells sharing similar signaling pathways. Cell, 112 (3): 293-301. [PMID:12581520]

To cite this database page, please use the following:

David E. Clapham, Bernd Nilius, Grzegorz Owsianik.
Transient Receptor Potential channels: TRPM5. Last modified on 28/02/2012. Accessed on 19/05/2013. IUPHAR database (IUPHAR-DB), http://www.iuphar-db.org/DATABASE/ObjectDisplayForward?objectId=497.


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