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Kir2.2

Family: Inwardly rectifying potassium channels

Contents:
Gene and Protein Information
Previous and Unofficial Names
Database Links
Associated Proteins
Ion Selectivity and Conductance
Gating Inhibitors
Tissue Distribution
Functional Assays
Physiological Functions
References
Gene and Protein Information
Species TM P Loops AA Chromosomal Location Gene Symbol Gene Name Reference
Human 2 1 433 17p11.1 KCNJ12 potassium inwardly-rectifying channel, subfamily J, member 12 4,14
Mouse 2 1 427 11 B2 Kcnj12 potassium inwardly-rectifying channel, subfamily J, member 12 11-12
Rat 2 1 427 10q22 Kcnj12 potassium inwardly-rectifying channel, subfamily J, member 12 6
Previous and Unofficial Names
IRK2
RB-IRK2
MB-IRK2
hIRK
Kir2.2
KCNJN1
Kir2.2v
hIRK1
potassium inwardly-rectifying channel, subfamily J, inhibitor 1
Kir2.1
MGC156606
ATP-sensitive inward rectifier potassium channel 12
IRK-2
inward rectifier K(+) channel Kir2.2
potassium channel, inwardly rectifying subfamily J member 12
potassium inwardly-rectifying channel, subfamily J, member 12
Database Links
DrugBank Target 1968 (Hs)
Ensembl ENSG00000184185 (Hs), ENSMUSG00000042529 (Mm), ENSRNOG00000002303 (Rn)
Entrez Gene 3768 (Hs), 16515 (Mm), 117052 (Rn)
GeneCards KCNJ12 (Hs)
GenitoUrinary Development Molecular Anatomy Project Kcnj12 (Mm)
HomoloGene 7793 (Hs)
Human Protein Reference Database 09083 (Hs)
InterPro Q14500 (Hs), P52187 (Mm), P52188 (Rn)
KEGG Gene hsa:3768 (Hs), mmu:16515 (Mm), rno:117052 (Rn)
OMIM 602323 (Hs)
PharmGKB Gene PA218 (Hs)
PhosphoSitePlus Q14500 (Hs), Q8CCR0 (Mm), P52188 (Rn)
Protein Ontology (PRO) PRO:000001981 (Hs)
RefSeq Nucleotide NM_021012 (Hs), NM_010603 (Mm), NM_053981 (Rn)
RefSeq Protein NP_066292 (Hs), NP_034733 (Mm), NP_446433 (Rn)
TreeFam ENSG00000184185 (Hs), ENSMUSG00000042529 (Mm), ENSRNOG00000002303 (Rn)
UniGene Hs. 200629 (Hs)
UniProt Q14500 (Hs), P52187 (Mm), P52188 (Rn)
Wikipedia Kir2.2
Potassium channels
Search for 3D structures on the PDB
Search by keyword: Inwardly rectifying potassium channels Kir2.2 Search by accession number: P52188, P52187, Q14500
Associated Proteins
Heteromeric Pore-forming Subunits
Name References
Kir2.1 9,15
Auxiliary Subunits
Name References
Not determined
Other Associated Proteins
Name References
chapsyn-110 7
veli-1 7-8
veli-3 7-8
α1-, β1-, β2-syntrophin 7
α-dystrobrevin-2 7
α-dystrobrevin-1 7
Dp71 7
dystrophin 7
actin-binding LIM protein 7
Dlg3 7
Dlg2 7
SAP-97 7-8
PSD-95 7
SAP102 7
CASK 7-8
Ion Selectivity and Conductance
Species:  Mouse
Rank order:  K+ [34.2 pS]
References:  11
Voltage Dependence
Not Determined
Activators
Not Determined
Gating inhibitors
Key to terms and symbols Click column headers to sort
Ligand Sp. Affinity Units Concentration range (M) Holding voltage (mV) Reference
Ba2+ Mm - - 5x10-5 -150.0 – -50.0 11
Cs+ Mm - - 5x10-6 - 5x10-5 -150.0 – -50.0 11
Ba2+ Rn 5.2 pKi - -80.0 13
View species-specific gating inhibitor tables
Tissue Distribution
Aortic endothelial cells.
Species:  Human
Technique:  Western Blot
References:  2
Heart, forebrain, cerebellum, skeletal muscle.
Species:  Mouse
Technique:  Northern Blot
References:  11
Cerebellum > motor trigeminal nucleus > olfactory bulb, piriform cortex, cerebral cortex, hippocampus, dentate gyrus, pontine nucleus.
Species:  Mouse
Technique:  In situ hybridisation
References:  3
Thalamus > cerebellum (granule cell layer), brainstem (inf. olivary , motor trigeminal, facial, cochlear and hypoglossal nuclei) > basal ganglia (habenula, vertical limb of the diagonal band) > superior colliculus.
Species:  Rat
Technique:  In situ hybridisation
References:  5
Medial habenula, spinal medulla > cortex, hippocampus, basal ganglia (caudate putamen, nucleus accumbens, globus pallidus), thalamus, substantia nigra, cerebellum > hypothalamus.
Species:  Rat
Technique:  Immunohistochemistry
References:  10
Functional Assays
Patch clamp measurement of the K+ current in cardiac myocytes of Kir2.2 knockout mice using patch clamp.
Species:  Mouse
Tissue:  Heart.
Response measured:  Reduction of IK1 current.
References:  15
Physiological Functions
Maintenance of the resting membrane potential and regulation of the excitability in neurons.
Species:  Mouse
Tissue:  Brain.
References:  1
Formation of the heart IK1 current, with Kir2.1.
Species:  Mouse
Tissue:  Heart.
References:  15

REFERENCES

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1. Day, M; Carr, DB; Ulrich, S; Ilijic, E; Tkatch, T; Surmeier, DJ. (2005) Dendritic excitability of mouse frontal cortex pyramidal neurons is shaped by the interaction among HCN, Kir2, and Kleak channels. J. Neurosci., 25 (38): 8776-87. [PMID:16177047]

2. Fang, Y; Schram, G; Romanenko, VG; Shi, C; Conti, L; Vandenberg, CA; Davies, PF; Nattel, S; Levitan, I. (2005) Functional expression of Kir2.x in human aortic endothelial cells: the dominant role of Kir2.2. Am. J. Physiol., Cell Physiol., 289 (5): C1134-44. [PMID:15958527]

3. Horio, Y; Morishige, K; Takahashi, N; Kurachi, Y. (1996) Differential distribution of classical inwardly rectifying potassium channel mRNAs in the brain: comparison of IRK2 with IRK1 and IRK3. FEBS Lett., 379 (3): 239-43. [PMID:8603697]

4. Hugnot, JP; Pedeutour, F; Le Calvez, C; Grosgeorge, J; Passage, E; Fontes, M; Lazdunski, M. (1997) The human inward rectifying K+ channel Kir 2.2 (KCNJ12) gene: gene structure, assignment to chromosome 17p11.1, and identification of a simple tandem repeat polymorphism. Genomics, 39 (1): 113-6. [PMID:9027495]

5. Karschin, C; Dissmann, E; Stühmer, W; Karschin, A. (1996) IRK(1-3) and GIRK(1-4) inwardly rectifying K+ channel mRNAs are differentially expressed in the adult rat brain. J. Neurosci., 16 (11): 3559-70. [PMID:8642402]

6. Koyama, H; Morishige, K; Takahashi, N; Zanelli, JS; Fass, DN; Kurachi, Y. (1994) Molecular cloning, functional expression and localization of a novel inward rectifier potassium channel in the rat brain. FEBS Lett., 341 (2-3): 303-7. [PMID:8137958]

7. Leonoudakis, D; Conti, LR; Anderson, S; Radeke, CM; McGuire, LM; Adams, ME; Froehner, SC; Yates, JR; Vandenberg, CA. (2004) Protein trafficking and anchoring complexes revealed by proteomic analysis of inward rectifier potassium channel (Kir2.x)-associated proteins. J. Biol. Chem., 279 (21): 22331-46. [PMID:15024025]

8. Leonoudakis, D; Conti, LR; Radeke, CM; McGuire, LM; Vandenberg, CA. (2004) A multiprotein trafficking complex composed of SAP97, CASK, Veli, and Mint1 is associated with inward rectifier Kir2 potassium channels. J. Biol. Chem., 279 (18): 19051-63. [PMID:14960569]

9. Preisig-Müller, R; Schlichthörl, G; Goerge, T; Heinen, S; Brüggemann, A; Rajan, S; Derst, C; Veh, RW; Daut, J. (2002) Heteromerization of Kir2.x potassium channels contributes to the phenotype of Andersen's syndrome. Proc. Natl. Acad. Sci. U.S.A., 99 (11): 7774-9. [PMID:12032359]

10. Prüss, H; Derst, C; Lommel, R; Veh, RW. (2005) Differential distribution of individual subunits of strongly inwardly rectifying potassium channels (Kir2 family) in rat brain. Brain Res. Mol. Brain Res., 139 (1): 63-79. [PMID:15936845]

11. Takahashi, N; Morishige, K; Jahangir, A; Yamada, M; Findlay, I; Koyama, H; Kurachi, Y. (1994) Molecular cloning and functional expression of cDNA encoding a second class of inward rectifier potassium channels in the mouse brain. J. Biol. Chem., 269 (37): 23274-9. [PMID:8083233]

12. Takumi, T; Tsuji, L; Kondo, C; Takahashi, N; Morishige, K; Copeland, NG; Gilbert, DJ; Jenkins, NA; Kurachi, Y. (1996) Assignment of the murine inward rectifier potassium channel Irk2 (Kir2.2) gene to the central region of mouse chromosome 11. Genomics, 37 (2): 270-2. [PMID:8921409]

13. Töpert, C; Döring, F; Wischmeyer, E; Karschin, C; Brockhaus, J; Ballanyi, K; Derst, C; Karschin, A. (1998) Kir2.4: a novel K+ inward rectifier channel associated with motoneurons of cranial nerve nuclei. J. Neurosci., 18 (11): 4096-105. [PMID:9592090]

14. Wible, BA; De Biasi, M; Majumder, K; Taglialatela, M; Brown, AM. (1995) Cloning and functional expression of an inwardly rectifying K+ channel from human atrium. Circ. Res., 76 (3): 343-50. [PMID:7859381]

15. Zaritsky, JJ; Redell, JB; Tempel, BL; Schwarz, TL. (2001) The consequences of disrupting cardiac inwardly rectifying K(+) current (I(K1)) as revealed by the targeted deletion of the murine Kir2.1 and Kir2.2 genes. J. Physiol. (Lond.), 533 (Pt 3): 697-710. [PMID:11410627]

To cite this database page, please use the following:

John P. Adelman, David E. Clapham, Hiroshi Hibino, Atsushi Inanobe, Lily Y. Jan, Andreas Karschin, Yoshihiro Kubo, Yoshihisa Kurachi, Michel Lazdunski, Takashi Miki, Colin G. Nichols, Wade L. Pearson, Susumu Seino, Carol A. Vandenberg.
Inwardly rectifying potassium channels: Kir2.2. Last modified on 01/07/2010. Accessed on 23/05/2013. IUPHAR database (IUPHAR-DB), http://www.iuphar-db.org/DATABASE/ObjectDisplayForward?objectId=431.


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